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High-sensitivity immunochromatographic assay for fumonisin B1 based on indirect antibody labeling

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Abstract

Objective

To develop a high-sensitivity immunochromatographic test for fumonisin B1 in plant extracts.

Results

Unlike conventional immunochromatographic tests, this assay is performed in two stages: competitive reaction with free specific antibodies and identifying immune complexes by their interaction with the anti-species antibody-conjugated gold nanoparticles. The use of a new geometry for the test strip membranes and a novel reagent application method ensures the proper order of these stages without additional manipulations. The contact of the ready-to-use test strip with the liquid sample suffices in initiating all stages of the assay and obtaining test results. The developed test was used on corn extracts; its instrumental limit of fumonisin B1 detection was 0.6 ng ml−1 at 15 min of assay duration.

Conclusions

The proposed approach is flexible and can be used for a wide range of low molecular compounds. The use of anti-species antibody-conjugated gold nanoparticles in immunochromatography significantly facilitates the development of test systems by eliminating the need to synthesize and characterize the conjugates with specific antibodies for each new compound to be detected.

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Acknowledgements

This study was financially supported by the Russian Foundation for Basic Research (Grant 15-08-07913).

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Authors and Affiliations

  1. A.N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 33 Leninsky prospect, 119071, Moscow, Russia

    Alexandr E. Urusov, Alina V. Petrakova, Milyausha K. Gubaydullina, Anatoly V. Zherdev, Sergei A. Eremin & Boris B. Dzantiev

  2. Department of Chemical Enzymology, Faculty of Chemistry, M.V. Lomonosov Moscow State University, 1 Leninskie Gory, 119991, Moscow, Russia

    Sergei A. Eremin

  3. School of Food Science and Technology, Jiangnan University, Wuxi, 214122, Jiangsu, China

    Dezhao Kong, Liqiang Liu & Chuanlai Xu

Authors
  1. Alexandr E. Urusov
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  2. Alina V. Petrakova
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  3. Milyausha K. Gubaydullina
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  4. Anatoly V. Zherdev
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  5. Sergei A. Eremin
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  6. Dezhao Kong
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  7. Liqiang Liu
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  8. Chuanlai Xu
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  9. Boris B. Dzantiev
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Corresponding author

Correspondence to Boris B. Dzantiev.

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Urusov, A.E., Petrakova, A.V., Gubaydullina, M.K. et al. High-sensitivity immunochromatographic assay for fumonisin B1 based on indirect antibody labeling. Biotechnol Lett 39, 751–758 (2017). https://doi.org/10.1007/s10529-017-2294-5

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  • DOI: https://doi.org/10.1007/s10529-017-2294-5

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