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Comparation of the effects of different 5′-untranslated regions (UTRs) on gene expression in HEK293 cells

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Abstract

Objectives

To evaluate four 5′-UTRs on GFP expression in HEK293T cells.

Results

The recombinant plasmids were constructed by restriction enzyme digestion, digestion and DNA sequencing. Quantitative real-time PCR and western blotting results showed that the transcription and translation level of PPRV-GFP mRNA was significantly lower than that of the other reporters. The transcription and translation level of ChEF1-GFP was the highest in HEK293T cells.

Conclusions

Different UTRs can significantly affect protein expression. Additionally, the findings also will be useful in biological applications that require tuning of gene expression and system optimization.

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Acknowledgments

This work was supported by the Genetically modified organisms breeding major projects (No. 2014ZX08010B15), the basic research projects of Henan province (142300413209), the Recommend International Advanced Agricultural Science and Technology Plan (“948” project) of China (No. 2011-G35).

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Authors and Affiliations

  1. Key Laboratory of Animal Biochemistry and Nutrition, Ministry of Agriculture, Henan Agricultural University, Zhengzhou, Henan, People’s Republic of China

    Xue-Mei Zhang, Guang-Ming Zha, Jiang Wang, Xin-Jian Wang, Shuang Song, Jing-Chao Shu, Bei-Bei Chu & Guo-Yu Yang

  2. College of Animal Husbandary and Veterinary Science, Henan Agricultural University, Wenhua Road 95, Zhengzhou, 450002, People’s Republic of China

    Guo-Yu Yang

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  1. Xue-Mei Zhang
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  2. Guang-Ming Zha
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  3. Jiang Wang
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  4. Xin-Jian Wang
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  5. Shuang Song
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  7. Bei-Bei Chu
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Correspondence to Guo-Yu Yang.

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Zhang, XM., Zha, GM., Wang, J. et al. Comparation of the effects of different 5′-untranslated regions (UTRs) on gene expression in HEK293 cells. Biotechnol Lett 38, 2051–2057 (2016). https://doi.org/10.1007/s10529-016-2199-8

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  • DOI: https://doi.org/10.1007/s10529-016-2199-8

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