Skip to main content
SpringerLink
Log in

Comparison of adult stem cells derived from multiple stem cell niches

  • OriginalPaper
  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Objectives

Adult stem cells (ASCs) have great potential for tissue regeneration; however, comparative studies of ASCs from different niches are required to understand the characteristics of each population for their potential therapeutic uses.

Results

We compared the proliferation, stem cell marker expression, and differentiation potential of ASCs from bone marrow, skin dermis, and adipose tissue. ASCs from bone marrow and skin dermis showed 50–100 % increased proliferation in comparison to the ASCs from adipose tissues. Furthermore, ASCs from each stem cell niche showed differential expression of stem cell marker genes, and preferentially differentiated into cell types of their tissue of origin.

Conclusion

Different characters of each ASC might be major factors for their effective use for therapeutics and tissue regeneration.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  • Bae S, Ahn JH, Park CW, Son HK, Kim KS, Lim NK, Jeon CJ, Kim H (2009) Gene and microRNA expression signatures of human mesenchymal stromal cells in comparison to fibroblasts. Cell Tissue Res 335:565–573

    Article  CAS  PubMed  Google Scholar 

  • Baksh D, Yao R, Tuan RS (2007) Comparison of proliferative and multilineage differentiation potential of human mesenchymal stem cells derived from umbilical cord and bone marrow. Stem Cells 25:1384–1392

    Article  CAS  PubMed  Google Scholar 

  • Baraniak PR, McDevitt TC (2010) Stem cell paracrine actions and tissue regeneration. Regen Med 5:121–143

    Article  PubMed  PubMed Central  Google Scholar 

  • Ben-David U, Benvenisty N (2011) The tumorigenicity of human embryonic and induced pluripotent stem cells. Nat Rev Cancer 11:268–277

    Article  CAS  PubMed  Google Scholar 

  • Caplan AI (1991) Mesenchymal stem cells. J Orthop Res 9:641–650

    Article  CAS  PubMed  Google Scholar 

  • Dodson MV, Hausman GJ, Guan L, Du M, Rasmussen TP, Poulos SP, Mir P, Bergen WG, Fernyhough ME, McFarland DC, Rhoads RP, Soret B, Reecy JM, Velleman SG, Jiang Z (2010) Skeletal muscle stem cells from animals I. Basic cell biology. Int J Biol Sci 6:465–474

    Article  PubMed  PubMed Central  Google Scholar 

  • Eslaminejad MB, Fani N, Shahhoseini M (2013) Epigenetic regulation of osteogenic and chondrogenic differentiation of mesenchymal stem cells in culture. Cell J 15:1–10

    CAS  PubMed  PubMed Central  Google Scholar 

  • Gimble JM, Katz AJ, Bunnell BA (2007) Adipose-derived stem cells for regenerative medicine. Circ Res 100:1249–1260

    Article  CAS  PubMed  Google Scholar 

  • Halfon S, Abramov N, Grinblat B, Ginis I (2011) Markers distinguishing mesenchymal stem cells from fibroblasts are downregulated with passaging. Stem Cells Dev 20:53–66

    Article  CAS  PubMed  Google Scholar 

  • Kim SE, Kim BK, Gil JE, Kim SK, Kim JH (2007) Comparative analysis of the developmental competence of three human embryonic stem cell lines in vitro. Mol Cells 23:49–56

    CAS  PubMed  Google Scholar 

  • Krause DS, Theise ND, Collector MI, Henegariu O, Hwang S, Gardner R, Neutzel S, Sharkis SJ (2001) Multi-organ, multi-lineage engraftment by a single bone marrow-derived stem cell. Cell 105:369–377

    Article  CAS  PubMed  Google Scholar 

  • Lenoir N (2000) Europe confronts the embryonic stem cell research challenge. Science 287:1425–1427

    Article  CAS  PubMed  Google Scholar 

  • Lunyak VV, Rosenfeld MG (2008) Epigenetic regulation of stem cell fate. Hum Mol Genet 17:R28–R36

    Article  CAS  PubMed  Google Scholar 

  • Morey L, Santanach A, Di Croce L (2015) Pluripotency and epigenetic factors in mouse embryonic stem cell fate regulation. Mol Cell Biol 35:2716–2728

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Reinisch A, Etchart N, Thomas D, Hofmann NA, Fruehwirth M, Sinha S, Chan CK, Senarath-Yapa K, Seo EY, Wearda T (2015) Epigenetic and in vivo comparison of diverse MSC sources reveals an endochondral signature for human hematopoietic niche formation. Blood 125:249–260

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Rodrigues RM, De Kock J, Branson S, Vinken M, Meganathan K, Chaudhari U, Sachinidis A, Govaere O, Roskams T, De Boe V (2014) Human skin-derived stem cells as a novel cell source for in vitro hepatotoxicity screening of pharmaceuticals. Stem Cells Dev 23:44–55

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Shim JH, Kang HH, Lee TR, Shin DW (2012) Enrichment and characterization of human dermal stem/progenitor cells using collagen type IV. J Dermatol Sci 67:202–205

    Article  CAS  PubMed  Google Scholar 

  • Takahashi K, Tanabe K, Ohnuki M, Narita M, Ichisaka T, Tomoda K, Yamanaka S (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131:861–872

    Article  CAS  PubMed  Google Scholar 

  • Toma JG, Akhavan M, Fernandes KJ, Barnabe-Heider F, Sadikot A, Kaplan DR, Miller FD (2001) Isolation of multipotent adult stem cells from the dermis of mammalian skin. Nat Cell Biol 3:778–784

    Article  CAS  PubMed  Google Scholar 

  • Tropepe V, Hitoshi S, Sirard C, Mak TW, Rossant J, van der Kooy D (2001) Direct neural fate specification from embryonic stem cells: a primitive mammalian neural stem cell stage acquired through a default mechanism. Neuron 30:65–78

    Article  CAS  PubMed  Google Scholar 

  • Villaron EM, Almeida J, Lopez-Holgado N, Alcoceba M, Sanchez-Abarca LI, Sanchez-Guijo FM, Alberca M, Perez-Simon JA, San Miguel JF, Del Canizo MC (2004) Mesenchymal stem cells are present in peripheral blood and can engraft after allogeneic hematopoietic stem cell transplantation. Haematologica 89:1421–1427

    PubMed  Google Scholar 

Download references

Acknowledgments

This paper was supported by the Semyung University Research Grant of 2015.

Supporting Information

Supplementary Table 1—Gene Name and Assay ID Number in RT-PCR Analysis.

Author information

Authors and Affiliations

  1. Department of Biomedical Sciences, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA, 19104, USA

    Dong-Hun Woo

  2. Department of Oriental Cosmetic Science, Semyung University, Science & Engineering Bldg/Room 313, 65 Semyung-ro, Jecheon, Chungbuk, 390-711, Republic of Korea

    Hyung Seo Hwang & Joong Hyun Shim

Authors
  1. Dong-Hun Woo
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hyung Seo Hwang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Joong Hyun Shim
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Joong Hyun Shim.

Ethics declarations

Conflict of interest

The authors state no conflict of interest.

Electronic supplementary material

Below is the link to the electronic supplementary material.

Supplementary material 1 (DOCX 14 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Woo, DH., Hwang, H.S. & Shim, J.H. Comparison of adult stem cells derived from multiple stem cell niches. Biotechnol Lett 38, 751–759 (2016). https://doi.org/10.1007/s10529-016-2050-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10529-016-2050-2

Keywords

Search

Navigation