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Enhanced production of porcine circovirus type 2 (PCV2) virus-like particles in Sf9 cells by translational enhancers

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Abstract

Objective

To investigate the effect of three translational enhancers for enhancing transgene expression in baculovirus expression vector system using GFP as a reporter gene and selected translational enhancers to increase porcine circovirus type 2 (PCV2) VLPs production.

Results

P10UTR (the 3′-untranslated region from the baculovirus p10 gene), Syn21 (a synthetic AT-rich 21-bp sequence) and P10UTR/Syn21 increased the GFP yield by 1.4-, 4- and 4.8-fold, respectively. While IVS (intron from Drosophila myosin heavy chain gene) decreased the GFP yield by 65 %. Moreover, the synergy of P10UTR/Syn21 increased the yield of PCV2 VLPs by 4.1 fold (45 μg/106 cells) compared with standard baculovirus vector.

Conclusion

The synergy of P10UTR/Syn21 is a potential strategy to improve the recombinant vaccine production besides PCV2 VLPs in BEVS.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (Grant No. 31101803/C1803) and Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences (Grant No. SKLVBF201405).

Supporting information

Supplementary Table 1—Primers used in this study.

Author information

Authors and Affiliations

  1. College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi, People’s Republic of China

    Yangkun Liu, Yuanyuan Zhang, Huafang Hao, Xiangjing Fu, Zengqi Yang & Enqi Du

  2. China-UK, Nanyang Normal University-Rothamsted Research Joint Laboratory of Insect Biology, Nanyang Normal University, Nanyang, People’s Republic of China

    Lunguang Yao

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  1. Yangkun Liu
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  2. Yuanyuan Zhang
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  3. Lunguang Yao
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  4. Huafang Hao
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  5. Xiangjing Fu
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  6. Zengqi Yang
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  7. Enqi Du
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Corresponding authors

Correspondence to Zengqi Yang or Enqi Du.

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Liu, Y., Zhang, Y., Yao, L. et al. Enhanced production of porcine circovirus type 2 (PCV2) virus-like particles in Sf9 cells by translational enhancers. Biotechnol Lett 37, 1765–1771 (2015). https://doi.org/10.1007/s10529-015-1856-7

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  • DOI: https://doi.org/10.1007/s10529-015-1856-7

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