Abstract
A sensitive electrochemiluminescence (ECL) biosensor for cholesterol detection based on multifunctional core–shell structured microspheres (Fe3O4@SiO2–Au@mpSiO2) is reported. This microsphere consisted of a core of silica-coated magnetite nanoparticle, an active transition layer of gold nanoparticles and a mesoporous silica shell. Scanning electron microscopy was employed to observe the morphology of the nanomaterials and transmission electron microscopy was used to further confirm the subtle structure of Fe3O4@SiO2–Au@mpSiO2. The microspheres possessed a large surface area that increased enzyme loading, and an active transition layer gold nanoparticles enhanced the ECL signal. They were used to immobilize cholesterol oxidase for cholesterol detection with a high sensitivity, low detection limit and wide linear range. The linear range was from 0.83 to 2.62 mM with a detection limit of 0.28 µM (S/N = 3). Moreover, the reproducibility, stability and selectivity of the biosensor were established.
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References
Ballesta-Claver J, Ametis-Cabello J, Morales-Sanfrutos J, Megía-Fernández A, Valencia-Mirón MC, Santoyo-González F, Capitán-Vallvey LF (2012) Electrochemiluminescent disposable cholesterol biosensor based on avidin–biotin assembling with the electroformed luminescent conducting polymer poly(luminol-biotinylated pyrrole). Anal Chim Acta 754:91–98
Batra N, Tomar M, Gupta V (2012) Realization of an efficient cholesterol biosensor using ZnO nanostructured thin film. Analyst 137:5854–5859
Chen YJ, Gao P, Wang RX, Zhu CL, Wang LJ, Cao MS, Jin HB (2009) Porous Fe3O4/SnO2 core/shell nanorods, synthesis and electromagnetic properties. J Phys Chem C 113:10061–10064
Chen YJ, Zhang F, Zhao GG, Fang XY, Jin HB, Gao P, Zhu CL, Cao MS, Xiao G (2010) Synthesis, multi-nonlinear dielectric resonance, and excellent electromagnetic absorption characteristics of Fe3O4/ZnO core/shell nanorods. J Phys Chem C 114:9239–9244
Deng YH, Qi DW, Deng CH, Zhang XM, Zhao DY (2008) Superparamagnetic high-magnetization microspheres with an Fe3O4@SiO2 core and perpendicularly aligned mesoporous SiO2 shell for removal of microcystins. J Am Chem Soc 130:28–29
Dhand C, Singh SP, Arya SK, Datta M, Malhotra BD (2007) Cholesterol biosensor based on electrophoretically deposited conducting polymer film derived from nano-structured polyaniline colloidal suspension. Anal Chim Acta 602:244–251
Gulfam M, Lee JM, Kim J, Lim DW, Lee EK, Chung BG (2011) Highly porous core-shell polymeric fiber network. Langmuir 27:10993–10999
Jiang XY, Wu YH, Mao XY, Cui XJ, Zhu LD (2011) Amperometric glucose biosensor based on integration of glucose oxidase with platinum nanoparticles/ordered mesoporous carbon nanocomposite. Sens Actuators B 153:158–163
Karjalainen S, Sewon L, Soderling E, Larsson B, Johansson I, Simell O, Lapinleimu H, Seppanen R (1997) Salivary cholesterol of healthy adults in relation to serum cholesterol concentration and oral health. J Dent Res 76:1637–1643
Ma GC, Yan XQ, Li YL, Xiao LP, Huang ZJ, Lu YP, Fan J (2010) Ordered nanoporous silica with periodic 30-60 nm pores as an effective support for gold nanoparticle catalysts with enhanced lifetime. J Am Chem Soc 132:9596–9597
Matharu Z, Arya SK, Singh SP, Gupta V, Malhotra BD (2009) Langmuir-Blodgett film based on MEH-PPV for cholesterol biosensor. Anal Chim Acta 634:243–249
Richter MM (2004) Electrochemiluminescence (ECL). Chem Rev 104:3003–3036
Safavi A, Farjami F (2011) Electrodeposition of gold–platinum alloy nanoparticles on ionic liquid–chitosan composite film and its application in fabricating an amperometric cholesterol biosensor. Biosens Bioelectron 26:2547–2552
Schwertmann U, Cornell RM (1991) Iron Oxides in the Laboratory. Preparation and characterization, 1st edn. VCH, Weinheim
Shih W-C, Yang M-C, Lin MS (2009) Development of disposable lipid biosensor for the determination of total cholesterol. Biosens Bioelectron 24:1679–1684
Xu SJ, Liu Y, Wang TH, Li JH (2010) Highly sensitive electrogenerated chemiluminescence biosensor in profiling protein kinase activity and inhibition using gold nanoparticle as signal transduction probes. Anal Chem 82:9566–9572
Zhang MH, Yuan R, Chai YQ, Chen SH, Zhong HA, Wang C, Cheng YF (2012) A biosensor for cholesterol based on gold nanoparticles-catalyzed luminol electrogenerated chemiluminescence. Biosens Bioelectron 32:288–292
Zhao MG, Huan JY, Zhou Y, Chen Q, Pan XH, He HP, Ye ZZ (2013) A single mesoporous ZnO/Chitosan hybrid nanostructure for a novel free nanoprobe type biosensor. Biosens Bioelectron 43:226–230
Acknowledgments
This work is supported by National Natural Science Foundation of China (21075100, 21275119), Ministry of Education of China (708073), Specialized Research Fund for the Doctoral Program of Higher Education (20100182110015), State Key Laboratory of Electroanalytical Chemistry (SKLEAC 2010009), Natural Science Foundation Project of Chongqing City (CSTC-2011BA7003) and Fundamental Research Funds for the Central Universities (XDJK2012A004).
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Zhang, J., Chen, S., Tan, X. et al. Highly sensitive electrochemiluminescence biosensors for cholesterol detection based on mesoporous magnetic core–shell microspheres. Biotechnol Lett 36, 1835–1841 (2014). https://doi.org/10.1007/s10529-014-1547-9
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DOI: https://doi.org/10.1007/s10529-014-1547-9