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Association of Genotypes of ANGPTL3 with Vitamin D and Calcium Concentration in Cardiovascular Disease

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Abstract

One of the leading causes of mortality worldwide is cardiovascular disease, which is influenced by some variables, including calcium and vitamin D. This study aimed to assess the relationship between Angiopoietin-Like 3 (ANGPTL3) gene polymorphisms with vitamin D and calcium levels in cardiovascular disease (CVD) patients. In this research, 1002 people participated. Participants' anthropometric parameters, and FBG, calcium, and vitamin D were assessed. Blood samples were used to extract DNA. Taqman®-based polymerase chain reaction (PCR) was used to conduct genetic analysis for the rs10789117 and rs17458195. Statistical analysis was applied to determine differences across subgroups and the relationship between polymorphisms and disease. Age, body mass index (BMI), fasting Blood Sugar (FBG), phenylalanine ammonia-lyase (PAL), and smoking history were significantly correlated with CVD. Vitamin D was statistically associated with rs10789117 and rs17458195 in non-CVD individuals. In the moderate group, individuals with the C allele in rs10789117 showed a tenfold increase in vitamin D deficiency compared to those with the A allele. However, in rs11207997, individuals with the T allele had 5 to 6 times higher vitamin D deficiency than those with the C allele in all groups. This research demonstrates the relationship between some ANGPTL3 gene polymorphisms and complement levels in CVD patients. It may be concluded that individuals carrying these variants would likely benefit from using vitamin D and calcium supplements to avoid CVD.

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Author notes

  1. Malihe Aghasizadeh and Mahmoud Ghanei have equally contributed first authors.

  2. Ebrahim Miri-Moghaddam and Majid Ghayour-Mobarhan have equally contributed corresponding authors.

Authors and Affiliations

  1. International UNESCO Center for Health-Related Basic Sciences and Human Nutrition, Mashhad University of Medical Sciences, Mashhad, Iran

    Malihe Aghasizadeh, Sahar Ghoflchi, Parisa Asadian-sohan, Mohammad Haghani & Majid Ghayour-Mobarhan

  2. Medical Genetics and Molecular Medicine Department, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Mahmoud Ghanei

  3. Student Research Committee, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

    Mahmoud Ghanei

  4. Cardiovascular Diseases Research Center, Birjand University of Medical Sciences, Birjand, Iran

    Tooba Kazemi & Ebrahim Miri-Moghaddam

  5. Social Determinants of Health Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

    Habibollah Esmaily

  6. Department of Biostatistics, School of Health, Mashhad University of Medical Sciences, Mashhad, Iran

    Habibollah Esmaily

  7. Metabolic Syndrome Research Center, Mashhad University of Medical Sciences, Mashhad, Iran

    Amir Avan

  8. Division of Medical Education, Brighton & Sussex Medical School, Falmer, Brighton, Sussex, BN19PH, UK

    Gordon A. Ferns

  9. Razi Clinical Research Development Unit, Faculty of Medicine Birjand University of Medical Sciences, Birjand, Iran

    Tooba Kazemi

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  1. Malihe Aghasizadeh
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  2. Mahmoud Ghanei
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  3. Sahar Ghoflchi
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  4. Parisa Asadian-sohan
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  10. Ebrahim Miri-Moghaddam
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Contributions

MA, MG, SG and PA-s: wrote the main manuscript text and MH, EB, TK and HE: prepared figures. AA, GAF, EM-M and MG-M: revised the manuscript. All authors reviewed the manuscript.

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Correspondence to Ebrahim Miri-Moghaddam or Majid Ghayour-Mobarhan.

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Aghasizadeh, M., Ghanei, M., Ghoflchi, S. et al. Association of Genotypes of ANGPTL3 with Vitamin D and Calcium Concentration in Cardiovascular Disease. Biochem Genet (2023). https://doi.org/10.1007/s10528-023-10533-3

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