Abstract
Long non-coding RNAs (lncRNAs) are associated with colorectal cancer (CRC); however, CRC-related linc00239 functions have not been fully elucidated. Prognostic analysis of patients with CRC with linc00239 overexpression was performed using data from The Cancer Genome Atlas database. Cell Counting Kit-8 and Transwell were used to determine linc00239 functions for CRC cells. The lncRNA–miRNA–mRNA interaction network was used to screen target miRNAs and mRNAs regulated by linc00239. Quantitative real-time polymerase chain reaction and western blotting were used to confirm the miRNA and mRNA expression. Furthermore, a miRNA inhibitor was transfected into CRC cells, and cell function was evaluated. Results indicated a high linc00239 expression in the tumor tissue of patients with CRC. Transfection of linc00239 siRNA into SW480 and LOVO cells decreased cell proliferation, cell migration, and invasion. MiR-182-5p/metadherin (MTDH) axis is a downstream pathway of linc00239. MTDH expression, the activity of cell proliferation, migration, and invasion, which were suppressed by linc00239 siRNA, were partially attenuated when linc00239 siRNA and miR-182-5p inhibitor were co-transfected into the CRC cells. Furthermore, miR-182-5p expression was decreased and MTDH expression was promoted in CRC tissues. Altogether, linc00239 may promote CRC development through the miR-182-5p/MTDH axis.
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Data Availability
The datasets generated and/or analysed during the current study are available in the [GEPIA] repository [http://gepia.cancer-pku.cn/detail.php?gene=linc00239], [miRanda] software [http://www.mirbase.org/] and mirTarbase repository [http://miRTarBase.cuhk.edu.cn/].
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The study was supported by the [Medical Science and Technology Research Foundation of Guangdong Province, China] (Grant Number [A2021326]).
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JG and TX performed experiments and contributed to interpretation of data; JG wrote the original daft; SZ contributed to the project administration, data analysis, and funding acquisition; All authors contributed to design of the work, revised manuscript, and approved the final manuscript.
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Guo, J., Xie, T. & Zhang, S. Linc00239 Promotes Colorectal Cancer Development via MicroRNA-182-5p/Metadherin Axis. Biochem Genet (2023). https://doi.org/10.1007/s10528-023-10510-w
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DOI: https://doi.org/10.1007/s10528-023-10510-w