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MiR-181c-5p Regulates Lung Adenocarcinoma Progression via Targeting PRKN

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Abstract

Accumulating evidence indicates that microRNAs (miRNAs) have a vital effect on lung adenocarcinoma. However, the contributions and possible mechanisms of miR-181c-5p to lung adenocarcinoma remain largely unclear. Our objective is to clarify the potential mechanism by which miR-181c-5p regulates lung adenocarcinoma progression. RT-qPCR was performed to determine the levels of miR-181c-5p in lung adenocarcinoma tissues and cells. CCK-8 and Transwell assays were conducted to evaluate the viability, migration, and invasion of H460 cells, respectively. The putative target association between miR-181c-5p and the Parkin gene (PRKN) was predicted using miRDB and confirmed by dual-luciferase reporter assay. MiR-181c-5p expression was found to be up-regulated in both lung adenocarcinoma tissues and cells. Suppression of miR-181c-5p resulted in the inhibition of viability, migration, and invasion in lung adenocarcinoma cells. PRKN level was negatively related to miR-181c-5p expression and mediated with the miR-181c-5p’s functions on lung adenocarcinoma progression. MiR-181c-5p regulates lung adenocarcinoma progression via targeting PRKN, indicating miR-181c-5p is expected to be a diagnostic and predictive marker for lung adenocarcinoma, providing new insights into the development of treatment strategies for lung adenocarcinoma.

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Data Availability

All data generated or analyzed during this study are included in this published article.

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Acknowledgements

We deeply appreciate the support of all participants.

Funding

Not applicable.

Author information

Authors and Affiliations

  1. Department of Oncology, The Second People’s Hospital of Liaocheng, Linqing, 252600, Shandong Province, China

    Jing Wang

  2. Liaocheng Cancer Prevention and Treatment Hospital, Liaocheng, 252000, Shandong Province, China

    Man Li

  3. Department of Obstetrics, Qingdao Central Hospital, No 127 Siliu South Road, Shibei District, Qingdao, 266013, Shandong, China

    Meigui Wang

  4. Department of Radiotherapy, Qingdao Municipal Hospital, Qingdao, 266011, Shandong Province, China

    Jing Yang

  5. Department of Proctology, Rizhao City Central Hospital, Rizhao, 276800, Shandong Province, China

    Deguang Li

  6. Department of Oncology, Yantai Municipal Laiyang Central Hospital Affiliated to Weifang Medical College, Yantai, 265200, Shandong Province, China

    Yunxia Hao

Authors
  1. Jing Wang
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  2. Man Li
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  3. Meigui Wang
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  4. Jing Yang
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  5. Deguang Li
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Contributions

JW and YH conceived and designed the study. ML and MW performed the literature search and data extraction. JY and DL analyzed the data. JW drafted the manuscript. YH finalized the manuscript. All the authors participated in the experiments and read and approved the final manuscript.

Corresponding author

Correspondence to Yunxia Hao.

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The authors state that there is no conflict of interest.

Ethical Approval

The experimental protocol was established, according to the ethical guidelines of the Helsinki Declaration and was approved by the Ethics Committee of the Yantai Laiyang Central Hospital.

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The analysis was performed according to the principles of the Helsinki Declaration. Written informed consent was obtained from all patients.

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Wang, J., Li, M., Wang, M. et al. MiR-181c-5p Regulates Lung Adenocarcinoma Progression via Targeting PRKN. Biochem Genet 62, 1103–1114 (2024). https://doi.org/10.1007/s10528-023-10459-w

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  • DOI: https://doi.org/10.1007/s10528-023-10459-w

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