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Long Non-Coding RNA Small Nucleolar RNA Host Gene 4 Induced by Transcription Factor SP1 Promoted the Progression of Nasopharyngeal Carcinoma Through Modulating microRNA-510-5p/Centromere Protein F Axis

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Abstract

Long non-coding RNAs (LncRNAs) are implicated with tumorigenesis and the development of nasopharyngeal carcinoma (NPC). Previous studies suggested that long non-coding RNA small nucleolar RNA host gene 4 (SNHG4) exerted oncogenic roles in various cancers. However, the function and molecular mechanism of SNHG4 in NPC have not been investigated. In our study, it was confirmed that the SNHG4 level was enriched in NPC tissues and cells. Functional assays indicated that SNHG4 depletion inhibited the proliferation and metastasis but promoted apoptosis of NPC cells. Furthermore, we identified miR-510-5p as a downstream gene of SNHG4 in NPC cells and SNHG4 upregulated CENPF expression by binding to miR-510-5p. Moreover, there was a positive (or negative) association between CENPF and SNHG4 (or miR-510-5p) expression in NPC. In addition, rescue experiments verified that CENPF overexpression or miR-510-5p silencing abrogated inhibitory effects on NPC tumorigenesis caused by SNHG4 deficiency. The study demonstrated that SNHG4 promoted NPC progression via miR-510-5p/CENPF axis, providing a novel potential therapeutic target for NPC treatments.

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Data Availability

The analyzed datasets generated during the present study are available from the corresponding author on reasonable request.

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Authors and Affiliations

  1. Department of Otorhinolaryngology, The Affiliated People’s Hospital of Ningbo University, No. 251 East Baizhang Road, Ningbo, 315000, Zhejiang, China

    Shao Zhou, Cheng Cao & Jiandao Hu

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  1. Shao Zhou
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  2. Cheng Cao
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  3. Jiandao Hu
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Contributions

SZ and CC designed this study and performed all the experiments. SZ and JH analyzed the data, prepared the figures and drafted the initial manuscript. SZ reviewed and revised the manuscript. All authors read and approved the final manuscript.

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Correspondence to Shao Zhou.

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This study was approved by the Ethics Committee of Yinzhou Hospital Affiliated to the Medical School of Ningbo University.

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Supplementary Information

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10528_2023_10351_MOESM1_ESM.tif

Supplementary file1 (TIF 329 KB) Supplementary Figure 1. Western blot showed the protein levels of E-cadherin and N-cadherin in SUNE1 and HONE1 cells transfected with shNC, sh#1, and sh#2. *p < 0.05.

10528_2023_10351_MOESM2_ESM.tif

Supplementary file2 (TIF 749 KB) Supplementary Figure 2. (A) SNHG4 overexpression efficiency in NPC cells were confirmed by RT-qPCR. (B) RT-qPCR showed SNHG4 expression in NPC cells transfected with shNC, shSP1, shSP1+pcDNA3.1-SNHG4. (C-H) CCK-8, colony formation, TUNEL, flow cytometry and transwell assays showed cell proliferation, apoptosis, migration and invasion abilities.

10528_2023_10351_MOESM3_ESM.tif

Supplementary file3 (TIF 504 KB) Supplementary Figure 3. Western blot showed the protein levels of E-cadherin and N-cadherin in different groups. *p < 0.05.

10528_2023_10351_MOESM4_ESM.tif

Supplementary file4 (TIF 722 KB) Supplementary Figure 4. miR-510-5p regulated NPC progression via CENPF. (A-F) The CENPF expression, proliferation, apoptosis, migration, and invasion of NPC cells transfected with NC mimics, miR-510-5p mimics, and miR-510-5p mimics+pcDNA3.1-CENPF were assessed.

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Zhou, S., Cao, C. & Hu, J. Long Non-Coding RNA Small Nucleolar RNA Host Gene 4 Induced by Transcription Factor SP1 Promoted the Progression of Nasopharyngeal Carcinoma Through Modulating microRNA-510-5p/Centromere Protein F Axis. Biochem Genet 61, 1967–1986 (2023). https://doi.org/10.1007/s10528-023-10351-7

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