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miR-122 Inhibits the Cervical Cancer Development by Targeting the Oncogene RAD21

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Abstract

Cervical cancer (CC) is one of the most frequently diagnosed tumors in female. miR-122 has been proved to be dominant in CC. The particular role of miR-122 in CC is unclear. Thus, we attempted to investigate the prognostic role of miR-122 in CC. We used the database of Kaplan–Meier curve plot. Growth and apoptosis of C33A cells were detected by CCK-8, colony formation assay, transwell assays and flow cytometry analysis. The target gene of miR-122 was identified using bioinformatics, q-PCR, western blot and luciferase assay. It showed that CC patients with overexpression of miR-122 have a better prognosis in the Kaplan–Meier plot database analysis. Overexpressed miR-122 inhibited the malignant growth and induced apoptosis of CC. miR-122 targeting of RAD21 cohesin complex component (RAD21) was identified using bioinformatics, Q-PCR, western blot and luciferase assay analyses. Moreover, we found miR-122 conduct its functions via RAD21 via the PI3K/AKT signaling pathway. Importantly, overexpression of RAD21 restored the roles of miR-122 in CC. Our data suggested that miR-122 could block malignant growth and promoted apoptosis by targeting RAD21 in CC. Our finding indicates miR-122 could potentially participate in the pathogenesis and be a biomarker or the potential therapeutic target of CC.

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Data Availability

All primary data presented in this study are available from the corresponding author upon reasonable request.

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Acknowledgements

Not applicable.

Funding

This work was supported by Grants from Jiangsu Province Maternal and Child Health Research Project No. F20013492; Xuzhou Science and Technology Bureau No. KC9138.

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Author notes

  1. Yanling Yang and Hongjuan Song have contributed equally to this work.

Authors and Affiliations

  1. Xuzhou Maternal and Child Health Hospital, Xuzhou Medical University, Xuzhou, 221000, China

    Yanling Yang, Yang Liu, Wei Liu, Chunyang Li, Yuan Liu & Hongjuan Song

  2. College of Medicine, University of Illinois at Chicago, Chicago, Illinois, USA

    Wenyang Hu

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  1. Yanling Yang
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  2. Yang Liu
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  3. Wei Liu
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  7. Hongjuan Song
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Contributions

Conception and design: HS; acquisition of data: HS; analysis and interpretation of data: YY, YL; writing, review, and/or revision of the manuscript: YY, YL, HS; administrative, technical, or material support: WL, CL, YL, WH; study supervision: HS.

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Correspondence to Hongjuan Song.

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All the research procedures strictly followed the REMARK and ethical review boards of Xuzhou Maternal and Child Health Hospital affiliated to Xuzhou Medical University.

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Yang, Y., Liu, Y., Liu, W. et al. miR-122 Inhibits the Cervical Cancer Development by Targeting the Oncogene RAD21. Biochem Genet 60, 303–314 (2022). https://doi.org/10.1007/s10528-021-10098-z

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  • DOI: https://doi.org/10.1007/s10528-021-10098-z

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