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Association Between the Polymorphism of Aldehyde Dehydrogenase 2 Gene and Cerebral Infarction in a Hakka Population in Southern China

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Abstract

Genetic factors play an important role in determining the susceptibility to ischemic stroke. Herein, we examined the association of an aldehyde dehydrogenase 2 (ALDH2) gene polymorphism with cerebral infarction. Patients with cerebral infarction (n = 963) and healthy controls (n = 921) were included. Genotyping was performed using gene chip platform analysis, and Sanger sequencing was used to confirm ALDH2 genotypes. The risk prediction of ALDH2 polymorphisms for cerebral infarction was examined under three genetic modes of inheritance. For males, ALDH2*2/*2 genotype was a significant risk factor for cerebral infarction in the co-dominant model (age-, smoking-, and drinking-adjusted OR 1.514, 95% CI 1.005–2.282, p = 0.047) and the recessive model (age-, smoking-, and drinking-adjusted OR 1.601, 95% CI 1.078–2.379, p = 0.020). However, for females, ALDH2*2/*2 genotype was a protective factor for cerebral infarction in the co-dominant model (age-, smoking-, and drinking-adjusted OR 0.450 95% CI 0.215–0.941, p = 0.034) and the recessive model (age-, smoking-, and drinking-adjusted OR 0.440, 95% CI 0.214–0.903, p = 0.025). Further, logistic regression analysis revealed that age, smoking, hypertension, hyperlipidemia, and hypercholesterolemia were significant risks for the presence of cerebral infarction. In conclusion, these findings support an association of ALDH2 gene polymorphisms with ischemic stroke in a Chinese Hakka population. In particular, homozygote ALDH2*2/*2 may be a risk factor for cerebral infarction in males, but contribute to reduced risk for cerebral infarction in females.

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Acknowledgements

The author would like to thank other colleagues who were not listed in the authorship of Clinical Core Laboratory and Center for Precision Medicine, Meizhou People's Hospital (Huangtang Hospital), Meizhou Hospital Affiliated to Sun Yat-sen University for their helpful comments on the manuscript.

Funding

This study was supported by the Medical Scientific Research Foundation of Guangdong Province through Grant Number A2017404 (to Jing-yuan Hou), the Science and Technology Program of Meizhou through Grant Number 2018B024 (to Jing-yuan Hou), and the Key Scientific and Technological Project of Meizhou People’s Hospital through Grant Number MPHKSTP-20170101 (to Zhi-xiong Zhong).

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Authors and Affiliations

  1. Clinical Core Laboratory, Meizhou People’s Hospital (Huangtang Hospital), Meizhou Hospital Affiliated To Sun Yat-Sen University, Meizhou, 514031, People’s Republic of China

    Jing-yuan Hou, Qiao-ting Deng, Su-dong Liu & Li-fang Lin

  2. Center for Cardiovascular Diseases, Meizhou People’s Hospital (Huangtang Hospital), Meizhou Hospital Affiliated To Sun Yat-Sen University, Meizhou, 514031, People’s Republic of China

    Zhi-xiong Zhong

  3. Guangdong Provincial Engineering and Technological Research Center for Molecular Diagnostics of Cardiovascular Diseases, Meizhou, 514031, People’s Republic of China

    Jing-yuan Hou, Zhi-xiong Zhong, Qiao-ting Deng, Su-dong Liu & Li-fang Lin

  4. Guangdong Provincial Key Laboratory of Precision Medicine and Clinical Translational Research of Hakka Population, Meizhou, 514031, People’s Republic of China

    Jing-yuan Hou, Zhi-xiong Zhong, Qiao-ting Deng, Su-dong Liu & Li-fang Lin

  5. Clinical Core Laboratory, Center for Precision Medicine, Meizhou People’s Hospital (Huangtang Hospital), Meizhou Hospital Affiliated To Sun Yat-Sen University, Meizhou, 514031, People’s Republic of China

    Jing-yuan Hou

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  1. Jing-yuan Hou
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Fig. S1

Aldehyde dehydrogenase 2 (ALDH2) polymorphisms (rs671; c.504G>A) by gene-chip testing. (A) Wild-type homozygote, GG genotype (ALDH2*1/*1). (B) Mutant heterozygote, GA genotype (ALDH2*1/*2). (C) Mutant homozygote, AA genotype (ALDH2*2/*2). Electronic supplementary material 1 (TIF 412 kb)

Fig. S2

DNA sequencing chromatogram of ALDH2 polymorphisms (rs671; c.504G>A). (A) Wild-type homozygote, GG genotype (ALDH2*1/*1). (B) Mutant heterozygote, GA genotype (ALDH2*1/*2). (C) Mutant homozygote, AA genotype (ALDH2*2/*2). Single nucleotide polymorphisms (SNPs) are indicated by arrows. Electronic supplementary material 2 (TIF 3373 kb)

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Hou, Jy., Zhong, Zx., Deng, Qt. et al. Association Between the Polymorphism of Aldehyde Dehydrogenase 2 Gene and Cerebral Infarction in a Hakka Population in Southern China. Biochem Genet 58, 322–334 (2020). https://doi.org/10.1007/s10528-020-09950-5

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