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Protective Effects of Intralipid and Caffeic Acid Phenethyl Ester (CAPE) on Hepatotoxicity and Pancreatic Injury Caused by Dichlorvos in Rats

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Abstract

The present study was aimed to the investigate the protective effects of caffeic acid phenethyl ester (CAPE) and intralipid (IL) on hepatotoxicity and pancreatic injury caused by acute dichlorvos (D) intoxication in rats. Forty-eight Wistar rats were randomly divided into seven groups each containing seven rats except control groups. The groups included control, D, CAPE, IL, D + CAPE, D + IL, and D + CAPE + IL. Total antioxidant status and total oxidative stress levels were measured by automated colorimetric assay. Tissues were evaluated using hematoxylin and eosin (H&E) staining. Tissues were analyzed with hematoxylin and eosin by using standard protocols. Also, Bcl-2, Bax and caspase-3 were evaluated by immunohistochemical method in liver tissue. Total oxidant status in control, CAPE, and IL groups were significantly lower, and total antioxidant status in the D + CAPE, D + IL, and D + IL + CAPE groups were significantly higher compared to the D group. CAPE and IL treatment decreased the apoptotic and mitotic cell count in liver tissue. Parenchymal necrosis caused by dichlorvos is observed in pancreas tissues of rats. Mild congestion and edema formation occurred in pancreas tissues following D + CAPE and D + IL therapies. These results indicate that CAPE and IL have the potential to decrease oxidative stress and hepatic and pancreatic injuries caused by acute dichlorvos intoxication. These drugs can be considered as a new method for supportive and protective therapy against pesticide intoxication.

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Abbreviations

CAPE:

Caffeic acid phenethyl ester

IL:

Intralipid

D:

Dichlorvos

WHO:

World Health Organization

OPPs:

Organophosphate pesticides

AchE:

Acetylcholinesterase

TAS:

Total antioxidant status

TOS:

Total oxidant status

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Authors and Affiliations

  1. Department of Pharmacology, School of Medicine, Mustafa Kemal University, Hatay, Turkey

    Harun Alp & Neslihan Pinar

  2. Department of Medical Physiology, School of Medicine, Mustafa Kemal University, Hatay, Turkey

    Recep Dokuyucu

  3. Department of Molecular Biochemistry and Genetics, School of Medicine, Mustafa Kemal University, Hatay, Turkey

    Recep Dokuyucu

  4. Department of Emergency Medicine, School of Medicine, Mustafa Kemal University, Hatay, Turkey

    Mustafa Sahan

  5. Department of General Surgery, School of Medicine, Mustafa Kemal University, Hatay, Turkey

    Cem Oruc

  6. Department of Medical Biochemistry, School of Medicine, Dicle University, Diyarbakır, Turkey

    Ibrahim Kaplan

  7. Department of Medical Pathology, School of Medicine, Medeniyet University, Istanbul, Turkey

    Serkan Senol & Ayse Bahar Ceyran

Authors
  1. Harun Alp
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  2. Neslihan Pinar
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  3. Recep Dokuyucu
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  4. Mustafa Sahan
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  6. Ibrahim Kaplan
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  7. Serkan Senol
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  8. Ayse Bahar Ceyran
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Correspondence to Recep Dokuyucu.

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Alp, H., Pinar, N., Dokuyucu, R. et al. Protective Effects of Intralipid and Caffeic Acid Phenethyl Ester (CAPE) on Hepatotoxicity and Pancreatic Injury Caused by Dichlorvos in Rats. Biochem Genet 54, 803–815 (2016). https://doi.org/10.1007/s10528-016-9757-6

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  • DOI: https://doi.org/10.1007/s10528-016-9757-6

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