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Glutathione S-Transferase Pi-Ile 105 Val Polymorphism and Susceptibility to T2DM in Population from Turabah Region of Saudi Arabia

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Abstract

Type 2 diabetes mellitus is characterized by chronic hyperglycemia and associated with oxidative stress resulting from accumulation of free radicals in body's tissues, which especially affects beta cells in pancreas and is an important factor in the development of diabetes and its complications. Glutathione S-transferases (GSTs) are a family of antioxidant enzymes that play important roles in decreasing ROS species and act as a kind of antioxidant defense. In a case–control study, we investigated the role of GSTP1 Ile105Val polymorphism in predisposition to T2DM in patients from Tarabah province, Saudi Arabia. The polymorphism was screened by PCR–RFLP in 90 T2DM patients and 87 healthy controls. The genotypes and alleles frequencies in cases and controls were assessed using Cochran–Armitage trend test and odds ratios (ORs), and 95 % confidence intervals (CIs) in different genetic models of inheritance were calculated. Our data indicate that G allele (Val) is associated with an increased risk for T2DM in this population in any combination (OR 4.101, 95 % CI 1.986–8.469, P = 0.00008). This indicates that individuals who are carriers for the mutant allele, either in homozygous (GG) or heterozygous (AG) state, are at fourfold higher risk for development of T2DM than other subjects in this population.

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Authors and Affiliations

  1. College of Applied Medical Sciences - Turabah, Taif University, Taif, Saudi Arabia

    Adil Mergani & Rasha Nabeel Zahran

  2. Department of Genetics, Faculty of Agriculture, Ain Sham University, Cairo, Egypt

    Ahmed Abdelkhalik Mansour

  3. National Center for Radiation Research and Technology (NCRRT), Cairo, Egypt

    Osama Mosailhy Saleh

  4. Department of Medical Biotechnology, College of Applied Medical Sciences -Turabah, Taif University, Taif, Saudi Arabia

    Ahmed Abdelkhalik Mansour

  5. Department of Biology, College of Applied Medical Sciences -Turabah, Taif University, Taif, Saudi Arabia

    Osama Mosailhy Saleh

  6. Department of Medical Laboratories, College of Applied Medical Sciences -Turabah, Taif University, Taif, Saudi Arabia

    Mukhtar Ahmed Mohammed

  7. Department of Medical Registry, College of Applied Medical Sciences -Turabah, Taif University, Taif, Saudi Arabia

    Adil Musa Mustafa

  8. Department of Cardiology, College of Applied Medical Sciences -Turabah, Taif University, Taif, Saudi Arabia

    Tamer Askar

Authors
  1. Adil Mergani
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  2. Ahmed Abdelkhalik Mansour
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  3. Tamer Askar
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  4. Rasha Nabeel Zahran
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  5. Adil Musa Mustafa
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  6. Mukhtar Ahmed Mohammed
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  7. Osama Mosailhy Saleh
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Correspondence to Adil Mergani.

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Mergani, A., Mansour, A.A., Askar, T. et al. Glutathione S-Transferase Pi-Ile 105 Val Polymorphism and Susceptibility to T2DM in Population from Turabah Region of Saudi Arabia. Biochem Genet 54, 544–551 (2016). https://doi.org/10.1007/s10528-016-9740-2

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  • DOI: https://doi.org/10.1007/s10528-016-9740-2

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