Polymerase chain reaction (PCR) and DNA melting methods are run on nucleic acid analyzers. We propose a method for recording melting signals using analyzers of the ANK series that decreases errors in determining the main parameter – the DNA melting temperature Tm. We propose a method for assessing the width of the melting interval ΔT as an additional specificity criterion for PCR products. Measurement errors have been evaluated: standard deviation (RMS) for Tm, ≤0.11°C; for ΔT, ≤0.06°C.
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Translated from Meditsinskaya Tekhnika, Vol. 55, No. 5, Sep.-Oct., 2021, pp. 23-25.
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Kurochkin, V.E., Belov, D.A., Belov, Y.V. et al. A Method for Determining Characteristic Parameters of DNA Melting in Nucleic Acid Analyzers. Biomed Eng 55, 333–336 (2022). https://doi.org/10.1007/s10527-022-10130-5
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DOI: https://doi.org/10.1007/s10527-022-10130-5