Experimental in vitro studies have demonstrated the possibility of use and the efficiency of the hepatocyte suspension as a biological material for the elimination of toxic compounds and normalization of metabolic processes during plasma dialysis using a bioartificial liver device. The developed circuits and bioreactor devices provide optimal conditions for normal functioning of the rat hepatocyte suspension for several hours required for cell dialysis.
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References
Zhao, L. F., Pan, X. P., and Li, L. J., “Key challenges to the development of extracorporeal bioartificial liver support systems,” Hepatobiliary Pancreat. Dis. Int., 11, No. 3, 243-249 (2012).
Ryabinin, V. E., “Problems and prospects of development of extra-corporeal liver support systems,” Biomed. Khim., 61, No. 5, 545-559 (2015).
Sajiki, T., Iwata, H., Paek, H. J., Tosha, T., Fujita, S., Ueda, Y., Park, Y. G., Zhu, B., Satoh, S., Ikai, I., Yamaoka, Y., and Ikada, Y., “Morphologic studies of hepatocytes entrapped in hollow fibers of a bioartificial liver,” ASAIO J., 46, No. 1, 49-55 (2000).
Seglen, P. O., Methods in Cell Biology, Academic Press, New York (1976), pp. 29-83.
Karuzina, I. I. and Archakov, A. I., Modern Methods in Biochemistry [in Russian], Meditsina, Moscow (1977), pp. 49-62.
Kochetov, G. A., Practical Manual of Enzymology [in Russian], Vysshaya Shkola, Moscow (1980).
Gabrielyan, N. I. and Lipatova, V. I., “Experience in the use of middle molecule content in blood as a diagnostic criterion in children with kidney diseases,” Lab. Delo, 19, No. 3, 138-140 (1984).
Belkin, A. L. and Osadchaya, L. P., “Measurement of ammonia concentration in small amounts of blood,” Lab. Delo, 12, No. 3, 177 (1977).
Korenman, I. M., Photometric Analysis. Methods for Organic Compound Determination [in Russian], Khimiya, Moscow (1970).
Ryabinin, V. E., Polevschikova, E. E., Suprin, V. I., and Egorov, A. P., “A device for albumin and cytosol detoxification,” Med. Tekh., No. 3, 14-17 (2014).
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Translated from Meditsinskaya Tekhnika, Vol. 51, No. 4, Jul.-Aug., 2017, pp. 5-7.
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Ryabinin, V.E., Polevschikova, E.E., Popkov, P.N. et al. Hepatocyte Suspension as a Biological Material for Extracorporeal Liver Support Systems. Biomed Eng 51, 234–238 (2017). https://doi.org/10.1007/s10527-017-9721-8
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DOI: https://doi.org/10.1007/s10527-017-9721-8