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Effects of Beauveria bassiana on the growth and reproductive rates of Nezara viridula

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Abstract

A laboratory colony of the southern green stink bug, Nezara viridula L. (Heteroptera: Pentatomidae) was used to evaluate the effect of two strains of Beauveria bassiana (Balsamo-Crivelli) Vuillemin (Hypocreales: Cordycipitaceae) on its growth and reproductive rates. Four concentrations (106, 107, 108, and 109 conidia g−1) of native (NI8) and commercial (GHA) entomopathogenic fungi alongside a water control were used. Cumulative oviposition and survival of nine groups (ten couple per group per concentration) were used to calculate the demographic parameters of this insect. Two computations were done based on total offspring (fertile and infertile eggs) and eggs with developed embryo (fertile eggs). Net reproductive rates (Ro) on insect sprayed with NI8 calculated based on total offspring showed a dose-dependent effect (72.55, 85.50, 58.15, and 37.60 females per newborn female) compared with water control (87.65). In populations sprayed with GHA, only the highest concentration (109 conidia g−1) was lower than control (27.15). The calculated Ro values based on fertile eggs were much lower as it was expected with 60.75, 61.45, 45.45, and 32.05 females per newborn female from lowest to highest concentrations of NI8 and 21.50 for GHA highest concentration compared with water control (78.45). These results demonstrated that both native and commercial strains affected embryo development, decreasing growth and reproductive rates of N. viridula populations. Further field testing is needed to evaluate the potential for in-field control.

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Acknowledgements

The work is supported by USDA-ARS Research Project# 6066-22000-090-00D-Insect Control and Resistance Management in Corn, Cotton, Sorghum, Soybean, and Sweet Potato, and Alternative Approaches to Tarnished Plant Bug Control in the Southern United States. The authors thank Tabatha Nelson and Kelnisha Westbrook – ARS-USDA-SIMRU for maintaining the Nezara viridula egg production, recognition, and classification. Thanks to Julianna Jojoa, LA summer student—Pollinator Health in Southern Crop Ecosystem Research Unit, for her valuable help entering data.

Funding

This work is supported by USDA-ARS Research Project # 6066–22000 = 090-00D.

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Authors and Affiliations

  1. USDA-ARS Southern Insect Management Research Unit, 141 Experimental Station Rd, P.O. Box 346, Stoneville, MS, 38776, USA

    Maribel Portilla, James P. Glover & Gadi V. P. Reddy

  2. Center for Disease Control and Prevention (CDC), Laboratory Sciences and Diagnostic Branch, 1600 Clinton Road, Atlanta, GA, 30329, USA

    Moukaram Tertuliano

  3. USDA-ARS Pollinator Health in Southern Crop Ecosystem Research Unit, 141 Experimental Station Rd, P.O. Box 346, Stoneville, MS, 38776, USA

    Katherine Parys

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  1. Maribel Portilla
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  2. Moukaram Tertuliano
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  3. Katherine Parys
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  4. James P. Glover
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  5. Gadi V. P. Reddy
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Correspondence to Maribel Portilla.

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Portilla, M., Tertuliano, M., Parys, K. et al. Effects of Beauveria bassiana on the growth and reproductive rates of Nezara viridula. BioControl (2024). https://doi.org/10.1007/s10526-024-10258-1

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