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The Role of δ2-Opioid Receptors in the Regulation of Tolerance of Isolated Cardiomyocytes to Hypoxia and Reoxygenation

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Hypoxia (20 min) and reoxygenation (30 min) were simulated on isolated rat cardiomyocytes to evaluate the cytoprotective effect of selective δ2-opioid receptor agonist deltorphin II, opioid receptor antagonist naloxone methiodide, μ-opioid receptor antagonist CTAP, κ-opioid receptor antagonist nor-binaltorphimine, ε1-opioid receptor antagonist BNTX, and δ2-opioid receptors naltriben. Deltorphin II was administered 5 min before reoxygenation, antagonists were administered 10 min before reoxygenation. The cytoprotective effect of deltorphin II was assessed by the number of cardiomyocytes survived after hypoxia/reoxygenation, as well as by the lactate dehydrogenase content in the incubation medium. It has been established that the cytoprotective effect of deltorphin II occurs at a concentration of 64 nmol/liter and is associated with activation of δ2-opioid receptors.

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Authors and Affiliations

  1. Cardiology Research Institute, Tomsk National Research Medical Center, Tomsk, Russia

    A. V. Mukhomedzyanov, S. V. Popov, N. V. Naryzhnaya & L. N. Maslov

  2. Branch of the Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences, Pushchino, Moscow region, Russia

    V. N. Azev

Authors
  1. A. V. Mukhomedzyanov
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  2. S. V. Popov
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  3. N. V. Naryzhnaya
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  4. V. N. Azev
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  5. L. N. Maslov
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Correspondence to A. V. Mukhomedzyanov.

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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 176, No. 10, pp. 428-431, October, 2023

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Mukhomedzyanov, A.V., Popov, S.V., Naryzhnaya, N.V. et al. The Role of δ2-Opioid Receptors in the Regulation of Tolerance of Isolated Cardiomyocytes to Hypoxia and Reoxygenation. Bull Exp Biol Med 176, 433–436 (2024). https://doi.org/10.1007/s10517-024-06041-y

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  • DOI: https://doi.org/10.1007/s10517-024-06041-y

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