The study involved 138 women aged 45-60 years in perimenopause (n=55) and postmenopause (n=83) with insomnia (main groups) and without it (control). The levels of reduced (GSH) and oxidized (GSSG) glutathione and glutathione peroxidase activity were determined in erythrocytes; activities of glutathione S-transferase and glutathione reductase were measured in blood serum. The differences with the control groups were found only in perimenopause: higher glutathione reductase activity and reduced GSSG level and GSH/GSSG ratio in women with insomnia (p<0.05). The results of the comparative analysis between the main groups showed lower glutathione reductase activity, increased GSSG level, and a decrease the GSH/GSSG ratio in the postmenopausal period compared with the perimenopause (p<0.05). These results can be used in choosing the tactics for complex therapy of insomnia in menopausal women to correct free radical homeostasis and prevention of oxidative stress.
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Machi JF, Dias Dda S, Freitas SC, de Moraes OA, da Silva MB, Cruz PL, Mostarda C, Salemi VM, Morris M, De Angelis K, Irigoyen MC. Impact of aging on cardiac function in a female rat model of menopause: role of autonomic control, inflammation, and oxidative stress. Clin. Interv. Aging. 2016;11:341-350. https://doi.org/10.2147/CIA.S88441
Gava G, Orsili I, Alvisi S, Mancini I, Seracchioli R, Meriggiola MC. Cognition, mood and sleep in menopausal transition: the role of menopause hormone therapy. Medicina (Kaunas). 2019;55(10):668. https://doi.org/10.3390/medicina55100668
Reimund E. The free radical flux theory of sleep. Med. Hypotheses. 1994;43(4):231-233. https://doi.org/10.1016/0306-9877(94)90071-x
Atrooz F, Salim S. Sleep deprivation, oxidative stress and inflammation. Adv. Protein Chem. Struct. Biol. 2020;119:309-336. https://doi.org/10.1016/bs.apcsb.2019.03.001
Semenova NV, Madaeva IM, Brichagina AS, Kolesnikova LI. Levels of advanced oxidation protein products in blood plasma of peri- and postmenopausal women with insomnia. Acta Biomedica Scientifica. 2021;6(1):69-74. Russian. https://doi.org/10.29413/ABS.2021-6.1.12
Hachul de Campos H, Brandão LC, D’Almeida V, Grego BH, Bittencourt LR, Tufik S, Baracat EC. Sleep disturbances, oxidative stress and cardiovascular risk parameters in postmenopausal women complaining of insomnia. Climacteric. 2006;9(4):312-319. https://doi.org/10.1080/13697130600871947
Semenova NV, Madaeva IM, Brichagina AS, Kolesnikov SI, Kolesnikova LI. 8-Hydroxy-2’-deoxyguanosine as an oxidative stress marker in insomnia. Bull. Exp. Biol. Med. 2021;171(3):384-387. https://doi.org/10.1007/s10517-021-05233-0
Semenova NV, Madaeva IM, Kolesnikov SI, Solodova EI, Kolesnikova LI. Insomnia in peri- and postmenopausal women: plasma lipids, lipid peroxidation and some antioxidant system parameters. Neuropsychiatry. 2018;8(5):1452-1460. https://doi.org/10.4172/Neuropsychiatry.1000477
Gulec M, Ozkol H, Selvi Y, Tuluce Y, Aydin A, Besiroglu L, Ozdemir PG. Oxidative stress in patients with primary insomnia. Prog. Neuropsychopharmacol. Biol. Psychiatry. 2012;37(2):247-251. https://doi.org/10.1016/j.pnpbp.2012.02.011
Liang B, Li YH, Kong H. Serum paraoxonase, arylesterase activities and oxidative status in patients with insomnia. Eur. Rev. Med. Pharmacol. Sci. 2013;17(18):2517-2522.
Wang L, Ahn YJ, Asmis R. Sexual dimorphism in glutathione metabolism and glutathione-dependent responses. Redox. Biol. 2020;31:101410. https://doi.org/10.1016/j.redox.2019.101410
Menopause and menopausal condition in Women: Clinical Recommendations. URL: https://naonob.ru/media/2018/07/07/1241338634/Menopauza_i_klimaktericheskoe_sostoyanie_u_zhenshhiny.pdf
Poluektov MG, Buzunov RV, Averbukh VM, Verbitskiy EV, Zakharov AV, Kel’manson IA, Korabel’nikova EA, Litvin AYu, Madaeva IM, Pal’man AD, Rusetskiy YuYu, Strygin KN, Yakupov EZ. Project of clinical recommendations on diagnosis and treatment of chronic insomnia in adults. Nevrol. Revmatol. Consilium Medicum. 2016;(2):41-51. Russian.
Hissin PJ, Hilf R. Fluorimetric method for determination of oxidized and reduced glutathione in tissues. Anal. Biochem. 1976;74(1):214-226. https://doi.org/10.1016/0003-2697(76)90326-2
Kolesnikova LI, Madaeva IM, Semenova NV, Suturina LV, Berdina ON, Sholohov LF, Solodova EI. Pathogenic role of melatonin in sleep disorders in menopausal women. Bull. Exp. Biol. Med. 2013;156(1):104-106. https://doi.org/10.1007/s10517-013-2289-8.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 173, No. 6, pp. 765-769, June, 2022
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Semenova, N.V., Madaeva, I.M., Brichagina, A.S. et al. Glutathione Component of Antioxidant Status in Menopausal Women with Insomnia. Bull Exp Biol Med 173, 775–778 (2022). https://doi.org/10.1007/s10517-022-05628-7
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DOI: https://doi.org/10.1007/s10517-022-05628-7