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Insulin Resistance in Experimental Type 1 Diabetes Mellitus

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Experimental type 1 diabetes mellitus (T1DM) was induced in rats by daily intraperitoneal injections of alloxan in a dose of 90 mg/kg for 4 days. For verification of insulin resistance, insulin tolerance test was performed in 2 weeks and the glucose utilization rate constant (KITT) was calculated. The rats demonstrated the main symptoms of T1DM: hypoinsulinemia, hyperglycemia, ketonemia, glucosuria, ketonuria, polydipsia, polyphagia, weight loss, and insulin resistance, as evidenced by a decrease in KITT. The serum content of free fatty acids and triacylglycerols significantly increased. The content of triacylglycerols increased in skeletal muscles and decreased in the liver. A negative linear correlation was found between KITT and triacylglycerol content in muscles. Thus, the development of insulin resistance in experimental T1DM in rats is associated with accumulation of triacylglycerols in skeletal muscles.

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References

  1. Cleland SJ, Fisher B.M, Colhoun H.M, Sattar N, Petrie J.R. Insulin resistance in type 1 diabetes: what is ‘double diabetes’ and what are the risks? Diabetologia. 2013;56(7):1462-1470. https://doi.org/10.1007/s00125-013-2904-2

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Cree-Green M, Bergman BC, Cengiz E, Fox LA, Hannon TS, Miller K, Nathan B, Pyle L, Kahn D, Tansey M, Tichy E, Tsalikian E, Libman I, Nadeau KJ. Metformin improves peripheral insulin sensitivity in youth with type 1 diabetes. J. Clin. Endocrinol. Metab. 2019;104(8):3265-3278. https://doi.org/10.1210/jc.2019-00129

    Article  PubMed  PubMed Central  Google Scholar 

  3. DiMeglio LA, Evans-Molina C, Oram RA. Type 1 diabetes. Lancet. 2018;391:2449-2462. https://doi.org/10.1016/S0140-6736(18)31320-5

    Article  PubMed  PubMed Central  Google Scholar 

  4. Folch J, Lees M, Sloane Stanley GH. A simple method for the isolation and purification of total lipides from animal tissues. J. Biol. Chem. 1957;226(1):497-509.

    Article  CAS  Google Scholar 

  5. Gregory JM, Cherrington AD, Moore DJ. The peripheral peril: injected insulin induces insulin insensitivity in type 1 diabetes. Diabetes. 2020;69(5):837-847. https://doi.org/10.2337/dbi19-0026

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Kilpatrick ES, Rigby AS, Atkin SL. Insulin resistance, the metabolic syndrome, and complication risk in type 1 diabetes: “double diabetes” in the Diabetes Control and Complications Trial. Diabetes Care. 2007;30(3):707-712. https://doi.org/10.2337/dc06-1982

    Article  CAS  PubMed  Google Scholar 

  7. Liu HY, Cao SY, Hong T, Han J, Liu Z, Cao W. Insulin is a stronger inducer of insulin resistance than hyperglycemia in mice with type 1 diabetes mellitus (T1DM). J. Biol. Chem. 2009;284(40):27 090-27 100. https://doi.org/10.1074/jbc.M109.016675

  8. Mohan IK, Das UN. Effect of L-arginine-nitric oxide system on the metabolism of essential fatty acids in chemical-induced diabetes mellitus. Prostaglandins Leukot. Essent. Fatty Acids. 2000;62(1):35-46. https://doi.org/10.1054/plef.1999.0122

    Article  CAS  PubMed  Google Scholar 

  9. Monzillo LU, Hamdy O. Evaluation of insulin sensitivity in clinical practice and in research settings. Nutr. Rev. 2003;61(12):397-412. https://doi.org/10.1301/nr.2003.dec.397-412

    Article  PubMed  Google Scholar 

  10. Paschou SA, Papadopoulou-Marketou N, Chrousos GP, Kanaka-Gantenbein C. On type 1 diabetes mellitus pathogenesis. Endocr. Connect. 2018;7(1):R38-R46. https://doi.org/10.1530/EC-17-0347

    Article  CAS  PubMed  Google Scholar 

  11. Stefan N, Kantartzis K, Häring HU. Causes and metabolic consequences of Fatty liver. Endocr. Rev. 2008;29(7):939-960. https://doi.org/10.1210/er.2008-0009

    Article  CAS  PubMed  Google Scholar 

  12. Wolf P, Fellinger P, Pfleger L, Smajis S, Beiglböck H, Gajdošík M, Anderwald CH, Trattnig S, Luger A, Winhofer Y, Krššák M, Krebs M. Reduced hepatocellular lipid accumulation and energy metabolism in patients with long standing type 1 diabetes mellitus. Sci. Rep. 2019;9(1):2576. https://doi.org/10.1038/s41598-019-39362-4

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Siberian State Medical University, Ministry of Health of the Russian Federation, Tomsk, Russia

    V. V. Ivanov, E. E. Buyko, A. A. Ufandeev, K. I. Nevskaya, A. P. Zima, T. V. Saprina & E. V. Udut

  2. E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine, Tomsk National Research Medical Center, Russian Academy of Sciences, Tomsk, Russia

    E. E. Buyko & V. V. Udut

Authors
  1. V. V. Ivanov
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  2. E. E. Buyko
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  3. A. A. Ufandeev
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  4. K. I. Nevskaya
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  5. V. V. Udut
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  6. A. P. Zima
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  7. T. V. Saprina
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  8. E. V. Udut
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Correspondence to V. V. Ivanov.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 172, No. 12, pp. 686-690, December, 2021

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Ivanov, V.V., Buyko, E.E., Ufandeev, A.A. et al. Insulin Resistance in Experimental Type 1 Diabetes Mellitus. Bull Exp Biol Med 172, 691–694 (2022). https://doi.org/10.1007/s10517-022-05458-7

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  • DOI: https://doi.org/10.1007/s10517-022-05458-7

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