The in vitro addition of water-soluble polysaccharides isolated from the leaves of Crataegus sanguinea Pall. to culture of mouse peritoneal macrophages induced classical activation of antigen-presenting cells by increasing NO synthase activity and reducing arginase expression.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Danilets MG, Bel’skaya NV, Bel’skaya YuP, Uchasova EG, Trofimova ES, Ligacheva AA, Gur’ev AM, Belousov MV, Akhmezhanov RR, Yusupov MS, Agafonov VI. The effect of plant polysaccharides on NO synthase and arginase of mouse macrophages. Vestn. Ural. Med. Akad. Nauki. 2009;(2):49-50. Russian.
Khasanova SR, Krivoshchekov SV, Kudashkina NV, Guryev AM, Rovkina KI, Belousov MV. Study on the polysaccharides of Crataegus sanguinea (rosaceae) leaves from flora of Bashkortostan republic. Rast. Resurs. 2015;51(3):397-406. Russian.
Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite, and [15N] nitrate in biological fluids Anal Biochem. 1982;126(1):131-138. doi: https://doi.org/10.1016/0003-2697(82)90118-x
Mosmann TR. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods. 1983;65(1-2):55-63. doi: https://doi.org/10.1016/0022-1759(83)90303-4
Munder M, Eichmann K, Modolell M. Alternative metabolic states in murine macrophages reflected by the nitric oxide synthase/arginase balance: competitive regulation by CD4+ T cells correlates with Th1/Th2 phenotype. J. Immunol. 1998; 160(11):5347-5354.
Rath M, Müller I, Kropf P, Closs EI, Munder M. Metabolism via arginase or nitric oxide synthase: two competing arginine pathways in macrophages. Front. Immunol. 2014;5:532. doi: https://doi.org/10.3389/fimmu.2014.00532
Schepetkin IA, Quinn MT. Botanical polysaccharides: macrophage immunomodulation and therapeutic potential. Int. Immunopharmacol. 2006;6(3):317-333. doi: https://doi.org/10.1016/j.intimp.2005.10.005
Shi L. Bioactivities, isolation and purification methods of polysaccharides from natural products: a review. Int. J. Biol. Macromol. 2016;92:37-48. doi: https://doi.org/10.1016/j.ijbiomac.2016.06.100
Wei W, Xiao HT, Bao WR, Ma DL, Leung CH, Han XQ, Ko CH, Lau CB, Wong CK, Fung KP, Leung PC, Bian ZX, Han QB. TLR-4 may mediate signaling pathways of Astragalus polysaccharide RAP induced cytokine expression of RAW264.7 cells. J. Ethnopharmacol. 2016;179:243-252.
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 172, No. 8, pp. 178-181, August, 2021
Rights and permissions
About this article
Cite this article
Ligacheva, A.A., Sherstoboev, E.Y., Danilets, M.G. et al. The Influence of Water-Soluble Polysaccharides of Crataegus sanguinea Pall. on Nitric Oxide Production by Macrophages. Bull Exp Biol Med 172, 151–154 (2021). https://doi.org/10.1007/s10517-021-05353-7
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-021-05353-7