The bactericidal activity of recombinant endolysins LysECD7, LysAm24, LysAp22, LysSi3 and LysSt11 was assayed in multidrug resistant strains (n=120) of Salmonella enterica, E. coli, Acinetobacter baumannii, Enterobacter spp., Pseudomonas aeruginosa, Klebsiella pneumoniae, and Campylobacter jejuni. The assay showed that the recombinant endolysins had a wide spectrum of bactericidal activity compared to endolysins of their progenitor phages. Among examined endolysins, we selected the active pharmaceutical substances with broad spectrum of bactericidal activity. Most strains were sensitive to LysECD7 (70.7%), LysAm24 (65%), and LysAp22 (58.6%), which seems to be promising causative agents for the development of finished dosage form.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Aleshkin AV, Volozhantsev NV, Svetoch EA, Kiseleva IA, Rubal’sky EO, Afanas’ev SS, Borzilov AI, Zatevalov AM, Vasil’ev DA, Zolotukhin SN, Zeigarnik MV, Galimzyanov KhM, Rubal’sky OV. Bacteriophages as probiotics: phagebased probiotic dietary supplement in prophylaxis against foodborne infections. Infekts. Bol. 2016;14(2):31-40. Russian.
Bochkareva SS, Karaulov AV, Aleshkin AV, Novikova II, Fedorova IM, Blayaher MS, Koteleva SI, Kapustin IV. Approaches to the estimation of some parameters of humoral and cellular immune response to bacteriophages. Klin. Lab. Diagnost. 2019;64(4):237-242. Russian.
Optimization of the system of measures to control and prevent infections associated with the provision of medical care in the Russian Federation. ORGZDRAV: Novosti, Mneniya, Obuchenie. Vestn. Vyssh, Shkoly Organizatsii Upravleniya Zravookhran. 2018;(1):17-26. Russian.
On Sanitary and Epidemiological Wellbeing of Population in Russian Federation in 2019: Government Report. Moscow, 2020. Russian.
Test VV, Tets GV. Microbial biofilms and Problems of antimicrobial therapy. Praktical. Pulmonol. 2013;(4):60-64. Russian.
Aleshkin AV, Ershova ON, Volozhantsev NV, Svetoch EA, Popova AV, Rubalskii EO, Borzilov AI, Aleshkin VA, Afanas’- ev SS, Karaulov AV, Galimzyanov KM, Rubalsky OV, Bochkareva SS. Phagebiotics in treatment and prophylaxis of healthcare- associated infections. Bacteriophage. 2016;6(4):e1251379. https://doi.org/10.1080/21597081.2016.1251379
Antonova NP, Balabanyan VY, Tkachuk AP, Makarov VV, Gushchin VA. Physical and chemical properties of recombinant KPP10 phage lysins and their antimicrobial activity against Pseudomonas aeruginosa. Bull. Russ. State Med. University. 2018;(1):21-27. https://doi.org/10.24075/brsmu.2018.010
Antonova NP, Vasina DV, Lendel AM, Usachev EV, Makarov VV, Gintsburg AL, Tkachuk AP, Gushchin VA. Broad bactericidal activity of the Myoviridae bacteriophage lysins LysAm24, LysECD7, and LysSi3 against gram-negative ESKAPE pathogens. Viruses. 2019;11(3). pii: E284. https://doi.org/10.3390/v11030284
Fernández-Ruiz I, Coutinho FH, Rodriguez-Valera F. Thousands of novel endolysins discovered in uncultured phage genomes. Front. Microbiol. 2018;9:1033. https://doi.org/10.3389/fmicb.2018.01033
Olsen NMC, Thiran E, Hasler T, Vanzieleghem T, Belibasakis GN, Mahillon J, Loessner MJ, Schmelcher M. Synergistic removal of static and dynamic Staphylococcus aureus biofilms by combined treatment with a bacteriophage endolysin and a polysaccharide depolymerase. Viruses. 2018;10(8). pii: E438. https://doi.org/10.3390/v10080438
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 170, No. 11, pp. 597-601, November, 2020
Rights and permissions
About this article
Cite this article
Vorob’ev, A.M., Anurova, M.N., Aleshkin, A.V. et al. Determination of Bactericidal Activity Spectrum of Recombinant Endolysins of ECD7, Am24, Ap22, Si3, and St11 Bacteriophages. Bull Exp Biol Med 170, 636–639 (2021). https://doi.org/10.1007/s10517-021-05122-6
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-021-05122-6