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Transforming Growth Factor Beta (TGF-β1) Induces Pro-Reparative Phenotypic Changes in Epicardial Cells in Mice

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Bulletin of Experimental Biology and Medicine Aims and scope

We evaluated the content of active form of TGF-β1 in the intact and post-infarction heart and the effect of this factor on the properties of epicardial cells. During the acute stage after myocardial infarction, the production of TGF-β1 in the heart increased, which closely correlated with activation of epicardial cells (appearance of a pool of Wt1+ epicardial cells entering the epithelial-mesenchymal transition). The role of TGF-β1 as the factor of epicardial activation was confirmed by the results of in vitro experiments: addition of recombinant TGF-β1 to cultured epicardial cells led to enhanced expression of genes of epithelial-mesenchymal transition and phenotypic transformation of these cells leading to the appearance of cells with markers of smooth muscle cells and fibroblasts. Our findings suggest that the regulatory axis “TGF-β1—epicardium cells” can be considered as an important link of the post-infarction reparative process and adaptive response during heart remodeling after myocardial infarction and as the target for therapeutic interventions.

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Authors and Affiliations

  1. Laboratory of Angiogenesis, Institute of Experimental Cardiology, National Medical Research Centre of Cardiology, Ministry of Health of the Russian Federation, Moscow, Russia

    K. V. Dergilev, Z. I. Tsokolaeva, I. B. Beloglazova, E. I. Ratner & E. V. Parfenova

  2. V. A. Negovsky Research Institute of General Reanimatology, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, Moscow, Russia

    Z. I. Tsokolaeva

  3. Laboratory of Postgenomic Technologies in Medicine, Faculty of Fundamental Medicine, M. V. Lomonosov Moscow State University, Moscow, Russia

    E. V. Parfenova

Authors
  1. K. V. Dergilev
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  2. Z. I. Tsokolaeva
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  3. I. B. Beloglazova
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  4. E. I. Ratner
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  5. E. V. Parfenova
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Correspondence to K. V. Dergilev.

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Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 4, pp. 278-283, December, 2020

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Dergilev, K.V., Tsokolaeva, Z.I., Beloglazova, I.B. et al. Transforming Growth Factor Beta (TGF-β1) Induces Pro-Reparative Phenotypic Changes in Epicardial Cells in Mice. Bull Exp Biol Med 170, 565–570 (2021). https://doi.org/10.1007/s10517-021-05107-5

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  • DOI: https://doi.org/10.1007/s10517-021-05107-5

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