Skip to main content
SpringerLink
Log in

Functional Imbalance of Glutamate- and GABAergic Neuronal Systems in the Pathogenesis of Focal Drug-Resistant Epilepsy in Humans

  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

The mechanisms of the formation of pharmacological resistance in temporal focal epilepsy remain poorly understood, and effective treatment strategies that can suppress epileptogenesis do not currently exist. We studied the imbalance between the glutamatergic (stimulating) and GABAergic (inhibitory) neuronal systems, as well as the role of apoptotic processes in the pathogenesis of drug-resistant epilepsy. To this end, the expression of Gad65, Vglut2, NR2B, Bcl-2, and caspase-8 proteins was analyzed in the gray and white matter of the temporal cortex of human brain. It was shown that pathological processes in the glutamatergic and GABAergic systems related to drug-resistant epilepsy are accompanied by changes in the content of apoptotic proteins, which can be the cause of neuronal death.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Aivazyan SO, Lukyanova EG, Shiryaev YuS. Modern treatment options for drugresistant epilepsy in children. Epilepsiya Paroksizmal. Sostoyaniya. 2014;6(1):34-43. Russian.

  2. Bazilevich SN. Objective factors of relative pharmacoresistance and possible causes of true pharmacoresistance in patients with epilepsy. Vestn. Ross. Voen.-Med. Akad. 2009;(2):118-123. Russian.

  3. Dorofeeva NA, Nikitina LS, Zosen DV, Glazova MV, Chernigovskaya EV. Functional state of the nigrostriatal system of Krushinsky–Molodkina rats during audiogenic seizure expression. Russ. J. Genetics: Appl. Res. 2017;7(3):217-225.

    Article  CAS  Google Scholar 

  4. Krylov VV, Guekht AB, Trifonov IS, Lebedeva AV, Kai-movsky IL, Sinkin MV, Grigorieva EV, Grishkina MN, Shyshkina LV, Kochetkova OO. Outcomes of surgical treatment of patients with pharmacoresistant epilepsy. Zh. Nevrol. Psikhiatr. 2016;116(9-2):13-18. doi: 10.17116/jnev-ro20161169213-18

  5. Sokolova TV, Zabrodskaya YM, Paramonova NM, Dobro-gorskaya LN, Kuralbaev AK, Kasumov VR, Sitovskaya DA. Apoptosis of brain cells in epileptic focus at phapmacresistant temporal lobe epilepsy computer modeling and epidemiology. Translyats. Med. 2017;4(6):22-33. Russian.

  6. Alsharafi WA, Xiao B, Li J. MicroRNA-139-5p negatively regulates NR2A-containing NMDA receptor in the rat pilocarpine model and patients with temporal lobe epilepsy. Epilepsia. 2016;57(11):1931-1940.

    Article  CAS  Google Scholar 

  7. Esclapez M, Houser CR. Up-regulation of GAD65 and GAD67 in remaining hippocampal GABA neurons in a model of temporal lobe epilepsy. J. Comp. Neurol. 1999;412(3):488-505.

    Article  CAS  Google Scholar 

  8. Löscher W. Molecular mechanisms of drug resistance in status epilepticu. Epilepsia. 2009;50(Suppl. 12):19-21. doi: https://doi.org/10.1111/j.1528-1167.2009.02367.x

Download references

Author information

Authors and Affiliations

  1. Laboratory of Morphology and Electron Microscopy, Institute of Toxicology, Federal Medical-Biological Agency of Russia, Moscow, Russia

    T. A. Sazhina, Yu. M. Zabrodskaya & E. D. Bazhanova

  2. Research Laboratory of Pathological Anatomy, A. L. Polenov Russian Research Neurosurgery Institute — Affiliated Branch of V. A. Almazov National Medical Research Center, Ministry of Health of the Russian Federation, Moscow, Russia

    D. A. Sitovskaya & Yu. M. Zabrodskaya

  3. Laboratory of Comparative Biochemistry of Cell Functions, I. M. Sechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of Sciences, St. Petersburg, Russia

    E. D. Bazhanova

  4. Joint Laboratory for Studies of the Role of Apoptosis in Neuroendocrine System Formation, Astrakhan State University, Astrakhan, Russia

    E. D. Bazhanova

Authors
  1. T. A. Sazhina
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. A. Sitovskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yu. M. Zabrodskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E. D. Bazhanova
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to E. D. Bazhanova.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 168, No. 4, pp. 519-522, October, 2019

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sazhina, T.A., Sitovskaya, D.A., Zabrodskaya, Y.M. et al. Functional Imbalance of Glutamate- and GABAergic Neuronal Systems in the Pathogenesis of Focal Drug-Resistant Epilepsy in Humans. Bull Exp Biol Med 168, 529–532 (2020). https://doi.org/10.1007/s10517-020-04747-3

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-020-04747-3

Keywords

Search

Navigation