Generation of ROS (including free radicals) in rat platelets during physical exercises of different intensities was evaluated by the chemiluminescent method. The acute or chronic submaximal exercises increased the platelet chemiluminescence by 2.5-3.0 times. In the control, platelet activation with physiological aggregant ADP enhanced their chemiluminescence by 7.5 times. Addition of ADP to platelets isolated from animals subjected to exercise of any intensity increased chemiluminescence by 4-5 times. In a trained organism, functional reserve of the platelets is preserved during long-term physical exercise of any intensity, but is exhausted after acute physical exercise. Thus, intensive physical exercises enhanced the hemostatic potential of the blood, which is associated with the risk of thrombohemorrhagic complications, especially in acute or submaximal long-term exercises.
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References
Vladimirov YuA, Proskurina EV. Free radicals and cell chemiluminescence. Uspekhi. Biol. Khimii. 2009;49:341-388. Russian.
Krivokhizhina LV, Ermolaeva EN, Surina-Marisheva EF, Kantyukov SA, Yakovleva VP. Chemiluminescence serum during exercise of varying intensity. Zdorov’e Obrazovanie XXI veke. 2016;18(2):542-547. Russian.
Krivokhizhina LV, Kantyukov SA, Ermolaeva EN, Krivokhizhin DN. Chemiluminescence of platelets. The use of chemiluminescence method to determine platelet activity. Vestn. Tyum. Gos. Univer. Med.-Biol. Nauki. 2013;(6):174-178. Russian.
Laboratory Methods of Study of Hemostatic System. Goldberg ED, ed. Tomsk, 1980. Russian.
Gorokhova VG, Kuznetsova EE, Gorokhov AG, Chashkova EJ, Korotaeva NS, Pak VE, Grigor’ev EG. Patent RU No. 2230322. Method of determining intracellular acidity. Bull. No. 16. Published June 10, 2004.
Taigunov MM, Farkhutdinov RR, Medvedev YuA. Chemiluminescent methods of blood analysis in clinical and experimental immunology. Med. Immunol. 2006;8(2-3):427-428. Russian.
Shitkova AS. Platelet Hemostasis. St. Petersburg, 2000. Russian.
Ersöz G, Zergeroglu AM, Fiçicilar H, Ozcan H, Oztekin P, Aytaç S, Yavuzer S. Effect of submaximal and incremental upper extremity exercise on platelet function and the role of blood shear stress. Thromb Res. 2002;108(5-6):297-301.
Freedman JE, Sauter R, Battinelli EM, Ault K, Knowles C, Huang PL, Loscalzo J. Deficient platelet-derived nitric oxide and enhanced hemostasis in mice lacking the NOSIII gene. Circ. Res. 1999;84(12):1416-1421.
Krötz F, Sohn HY, Pohl U. Reactive oxygen species: players in the platelet game. Arterioscler. Thromb. Vasc. Biol. 2004;24(11):1988-1996.
Tozzi-Ciancarelli MG, Penco M, Di Massimo C. Influence of acute exercise on human platelet responsiveness: possible involvement of exercise-induced oxidative stress. Eur. J. Appl. Physiol. 2002;86(3):266-272.
Wang JS, Liao CH. Moderate-intensity exercise suppresses platelet activation and polymorphonuclear leukocyte interaction with surface-adherent platelets under shear flow in men. Thromb. Haemost. 2004;91(3):587-594.
Weber AA, Heim HK, Schumacher M, Schrör K, Hohlfeld T. Effects of selective cyclooxygenase isoform inhibition on systemic prostacyclin synthesis and on platelet function at rest and after exercise in healthy volunteers. Platelets. 2007;18(5):379-385.
Whittaker JP, Linden MD, Coffey VG. Effect of aerobic interval training and caffeine on blood platelet function. Med. Sci. Sports Exerc. 2013;45(2):342-350.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 167, No. 6, pp. 686-689, June, 2019
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Sergienko, V.I., Kantyukov, S.A., Ermolaeva, E.N. et al. Platelet Chemiluminescence during Physical Exercise of Various Intensity. Bull Exp Biol Med 167, 732–734 (2019). https://doi.org/10.1007/s10517-019-04610-0
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DOI: https://doi.org/10.1007/s10517-019-04610-0