The contractile function of the heart was studied in adult frogs Rana temporaria under the influence of a toxic dose of isoprenaline under conditions of natural sinoatrial rhythm and during heart pacing. The dynamics of ventricular pressure was recorded with a Prucka MacLab 2000 instrument via a catheter introduced into the ventricle through the ventricular wall. Reduced (p<0.05) parameters of the pump function (HR, maximum ventricular systolic pressure, isovolumic indices dP/dtmax and dP/dtmin) and lengthening of QRS complex and QT interval on ECG attested to impairment of contractile function and electrical processes after exposure to isoprenaline. Electrical stimulation of the right atrium improved myocardial contractility and ECG parameters after the administration of isoprenaline.
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Bramich NJ, Cousins HM, Edwards FR, Hirst GD. Parallel metabotropic pathways in the heart of the toad, Bufo marinus. Am. J. Physiol. Heart Circ. Physiol. 2001;281(4):H1771-H1777.
Burggren WW, Vitalis TZ. The interplay of cutaneous water loss, gas exchange and blood flow in the toad, Bufo woodhousei: adaptations in a terrestrially adapted amphibian. J. Exp. Biol. 2005;208(Pt 1):105-112.
Cano-Martínez A, Vargas-González A, Guarner-Lans V. Temperature effect on the contractile activity of the Ambystoma dumerilii heart previously treated with isoproterenol. Comp. Biochem. Physiol. A Mol. Integr. Physiol. 2007;147(3): 743-749.
Jensen B, Wang T, Christoffels VM, Moorman AF. Evolution and development of the building plan of the vertebrate heart. Biochim. Biophys. Acta. 2013;1833(4):783-794.
Kibler NA, Nuzhny VP, Achmetzhynova SV, Shmakov DN. Effects of heart rate on the pump function and electrophysiological characteristics of the heart in the frog Rana temporaria. Int. J. Biomed. 2017;7(1):46-50. doi: https://doi.org/10.21103/Article7(1)_OA5.
Kibler NA, Nuzhny VP, Prosheva VI. Electrical and contractile properties of the heart ventricle in response to ambient temperature changes in frog Rana temporaria. Int. J. Biomed. 2017;7(3):180-184. doi: https://doi.org/10.21103/Article7(3)_OA4.
Lyon AR, Rees PS, Prasad S, Poole-Wilson PA, Harding SE. Stress (Takotsubo) cardiomyopathy — a novel pathophysiological hypothesis to explain catecholamine-induced acute myocardial stunning. Nat. Clin. Pract. Cardiovasc. Med. 2008; 5(1):22-29.
Maron BJ, Towbin JA, Thiene G, Antzelevitch C, Corrado D, Arnett D, Moss AJ, Seidman CE, Young JB; American Heart Association; Councilon Clinical Cardiology, Heart Failure and Transplantation Committee; Quality of Care and Outcomes Research and Functional Genomicsand Translational Biology Interdisciplinary Working Groups; Councilon Epidemiology and Prevention. Contemporary definitions and classification of the cardiomyopathies: an American Heart Association Scientific Statement from the Council on Clinical Cardiology, Heart Failure and Transplantation Committee; Quality of Care and Outcomes Research and Functional Genomics and Translational Biology Interdisciplinary Working Groups; and Council on Epidemiology and Prevention. Circulation. 2006;113(14):1807-1816.
Ostádal B, Pelouch V, Ostádalová I, Nováková O. Structural and biochemical remodelling in catecholamine-induced cardiomyopathy: comparative and ontogenetic aspects. Mol. Cell. Biochem. 1995;147(1-2):83-88.
Perry SF, Capaldo A. The autonomic nervous system and chromaffin tissue: neuroendocrine regulation of catecholamine secretion in non-mammalian vertebrates. The autonomic nervous system and chromaffin tissue: neuroendocrine regulation of catecholamine secretion in non-mammalian vertebrates. Auton. Neurosci. 2011;165(1):54-66.
Rocha PL, Branco LG. Cardiovascular, respiratory and metabolic resposes to temperature and hypoxia of the winter frog Rana catesbeiana. Braz. J. Med. Biol. Res. 1997;30(1):125-131.
Rocha PL, Branco LG. Seasonal changes in the cardiovascular, respiratory and metabolic responses to temperature and hypoxia in the bullfrog Rana catesbeiana. J. Exp. Biol. 1998;201(Pt5):761-768.
Samuels MA. The brain-heart connection. Circulation. 2007; 116(1):77-84.
Tota B, Cerra MC, Gattuso A. Catecholamines, cardiac natriuretic peptides and chromogranin A: evolution and physiopathology of a “whip-brake” system of the endocrine heart. J. Exp. Biol. 2010;213:3081-3103.
Volkmann R. Electrical and mechanical activity of isoproterenol- damaged frog heart. Comp. Biochem. Physiol. C. 1985;81(1):189-194.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 165, No. 5, pp. 545-549, May, 2018
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Kibler, N.A., Nuzhny, V.P. & Shmakov, D.N. Isoprenaline Impairs Contractile Function of Ventricular Myocardium in Common Frog (Rana temporaria). Bull Exp Biol Med 165, 606–609 (2018). https://doi.org/10.1007/s10517-018-4223-6
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DOI: https://doi.org/10.1007/s10517-018-4223-6