Skip to main content
SpringerLink
Log in

In Vitro Effects of Sodium Nitroprusside and L-Nω-Nitroarginine Methyl Ester (L-NAME) on Activity of Lysosomal Cysteine Proteinases and Lysosomal Membrane Permeability

  • Published:
Bulletin of Experimental Biology and Medicine Aims and scope

The direct effect of 5 mM L-NAME and 0.1 mM sodium nitroprusside on activity of lysosomal cysteine proteinases and permeability of lysosomal membrane was studied in vitro after 1, 2, and 4 h of incubation. Isolated from the liver of intact female rats lysosome suspensions were used. Both substances reduced total activity of cathepsin H and did not affect cathepsin B at all time intervals. L-NAME increased cathepsin L activity at all incubation times, while sodium nitroprusside increased activity of this enzyme after 2-h incubation and reduced it incubation after 4-h incubation. L-NAME demonstrated a membrane-destabilizing effect in in vitro experiments, while sodium nitroprusside on the contrary stabilized lysosomal membranes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Abalenihina JuV, Fomina MA. The impact of modulators of the synthesis of nitric oxide in the activity, autoprocessing cathepsin in immunocompetent organs of rats in conditions of in vitro. Nauka Molodykh — Eruditio Juvenium. 2014;(1):53-59. Russian.

    Google Scholar 

  2. Abalenihina JV, Fomina MA, Isakov SA. Oxidizing updating of proteins and change of activity cathepsin l rats spleen in the conditions of modeling deficiency of nitric oxide synthesis. Ross. Med.-Biol. Vestn. 2013;(1):44-48. Russian.

    Google Scholar 

  3. Arapova AI, Fomina MA. Effect of L-arginine and L-name on lysosomal cysteine proteases activity and lysosomal membranes permeability in rat aorta. Kazan. Med. Zh. 2016;97(2):250-255. Russian.

    Article  Google Scholar 

  4. Liga AB, Ukhina TV, Shimanovski NL. Activity lysosomal enzymes in rat skin fibroblasts after treatment with progesterone and new gestagen ABMP. Bull. Exp. Biol. Med. 2008;145(1):44-46.

    Article  PubMed  CAS  Google Scholar 

  5. Metelskaya VA, Gumanova NG. Screening as a method for determining the serum level of nitric oxide metabolites. Klin. Lab. Diagnost. 2005;(6):15-18. Russian.

  6. Pokrovskii AA, Tutel’yan VA. Lysosomes. Moscow, 1976. Russian.

  7. Pupyshev AB. Lysosomal membrane permeabilization as apoptogenic factor. Tsitologiya. 2011;53(4):313-324. Russian.

    CAS  Google Scholar 

  8. Barrett AJ, Kirschke H. Cathepsin B, cathepsin H, cathepsin L. Methods Enzymol. 1981;80( Pt C):535-561.

    Article  PubMed  CAS  Google Scholar 

  9. Fan H, Guo S, Jiao Y, Zhang R, Li J. Effects of exogenous nitric oxide on growth, active oxygen species metabolism, and photosynthetic characteristics in cucumber seedlings under NaCl stress. Front. Agric. China. 2007;1(3):308-314. doi: https://doi.org/10.1007/s11703-007-0052-5.

    Article  Google Scholar 

  10. Felbor U, Dreier L, Bryant RA, Ploegh HL, Olsen BR, Mothes W. Secreted cathepsin L generates endostatin from collagen XVIII. EMBO J. 2000;19(6):1187-1194.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  11. Fujishima A, Imai Y, Nomura T, Fujisawa Y, Yamamoto Y, Sugawara T. The crystal structure of human cathepsin L complexed with E-64. FEBS Lett. 1997;407(1):47-50.

    Article  PubMed  CAS  Google Scholar 

  12. Hottinger DG, Beebe DS, Kozhimannil T, Prielipp RC, Belani KG. Sodium nitroprusside in 2014: A clinical concepts review. J. Anaesthesiol. Clin. Pharmacol. 2014;30(4):462-471.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  13. Kaur G, Singh HP, Batish DR, Mahajan P, Kohli RK, Rishi V. Exogenous nitric oxide (NO) interferes with lead (Pb)-induced toxicity by detoxifying reactive oxygen species in hydroponically grown wheat (triticum aestivum) roots. PLoS One. 2015;10(9):e0138713. doi: https://doi.org/10.1371/journal.pone.0138713.

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  14. Terman A, Kurz T, Gustafsson B, Brunk UT. Lysosomal labilization. IUBMB Life. 2006;58(9):531-539.

    Article  PubMed  CAS  Google Scholar 

  15. Turk V, Stoka V, Vasiljeva O, Renko M, Sun T, Turk B, Turk D. Cysteine catepsines: from structure, function and regulation to new frontiers. Biochim. Biophys. Acta. 2012;1824(1):68-88.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. I. P. Pavlov Ryazan State Medical University, Ministry of the Health of the Russian Federation, Ryazan, Russia

    M. A. Fomina, A. M. Kudlaeva, S. A. Isakov & V. V. Davydov

Authors
  1. M. A. Fomina
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. M. Kudlaeva
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. A. Isakov
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. V. V. Davydov
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to A. M. Kudlaeva.

Additional information

Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 165, No. 1, pp. 43-46, January, 2018

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Fomina, M.A., Kudlaeva, A.M., Isakov, S.A. et al. In Vitro Effects of Sodium Nitroprusside and L-Nω-Nitroarginine Methyl Ester (L-NAME) on Activity of Lysosomal Cysteine Proteinases and Lysosomal Membrane Permeability. Bull Exp Biol Med 165, 36–39 (2018). https://doi.org/10.1007/s10517-018-4093-y

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10517-018-4093-y

Key Words

Search

Navigation