Depolarization of cardiomyocytes triggered by stretch and activation of mechanically gated ion channels can lead to serious arrhythmias. However, stretch-induced signaling activating these channels remain little studied. This study tested the hypothesis on implication of NO in shaping the electrical abnormalities provoked by stretch of the right atrial myocardium in rat via a mechanism engaging a signaling cascade, where NO plays a significant role. This approach showed that in isolated right atrial preparation, NO donor SNAP induces the electrical abnormalities similar to those provoked by stretch, and the latter results from activation of NO synthase.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Abramochkin DV, Kuzmin VS, Mitrochin VM, Kalugin L, Dvorzhak A, Makarenko EY, Schubert R, Kamkin A. TNF-α provokes electrical abnormalities in rat atrial myocardium via a NO-dependent mechanism. Pflugers Arch. 2013;465(12):1741-1752.
Kamkin A, Kiseleva I, Wagner KD, Leiterer KP, Theres H, Scholz H, Günther J, Lab MJ. Mechano-electric feedback in right atrium after left ventricular infarction in rats. J. Mol. Cell. Cardiol. 2000;32(3):465-477.
Mechanosensitivity in Cells and Tissues. Vol. 3. Mechanosensitivity of the Heart. Kamkin A, Kiseleva I, eds. London; New York, 2010. doi: https://doi.org/10.1007/978-90-481-2850-1.
Kazanski VE, Kamkin AG, Makarenko EY, Lysenko NN, Sutiagin PV, Kiseleva IS. Role of nitric oxide in the regulation of mechanosensitive ionic channels in cardiomyocytes: contribution of NO-synthases. Bull. Exp. Biol. Med. 2000;150(2):263-267.
Kazanski V, Mitrokhin VM, Mladenov MI, Kamkin AG. Cytokine Effects on Mechano-Induced Electrical Activity in Atrial Myocardium. Immunol. Invest. 2017;46(1):22-37.
Lammerding J, Kamm RD, Lee RT. Mechanotransduction in cardiac myocytes. Ann. N.Y. Acad. Sci. 2004;1015:53-70.
Mitrokhin VM, Mladenov MI, Kamkin AG. Effects of interleukin-6 on the bio-electric activity of rat atrial tissue under normal conditions and during gradual stretching. Immunobiology. 2015;220(9):1107-1112.
Casadei B, Sears CE. Nitric-oxide-mediated regulation of cardiac contractility and stretch responses. Prog. Biophys. Mol. Biol. 2003;82(1-3):67-80.
Tasaki K, Tsukahara J, Ito S, Wayner MJ, Yu WY. A simple, direct and rapid method for filling microelectrodes. Physiol. Behav. 1968;3(6):1009-1010.
Ward ML, Allen DG. Stretch-Activated Channels in the Heart: Contribution to Cardiac Performance. Mechanosensitivity in Cells and Tissues. Vol. 3. Mechanosensitivity of the Heart. London; New York, 2010. P. 141-167.
Zhang YH, Youm JB, Sung HK, Lee SH, Ryu SY, Ho WK, Earm YE. Stretch-activated and background non-selective cation channels in rat atrial myocytes. J. Physiol. 2000;523(Pt 3):607-619.
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 163, No. 6, pp. 668-673, June, 2017
Rights and permissions
About this article
Cite this article
Shim, A.L., Mitrokhin, V.M., Kazanski, V.E. et al. Discrete Stretch Eliminates Electrophysiological Dose-Dependent Effects of Nitric Oxide Donor SNAP in Rat Atrium. Bull Exp Biol Med 163, 705–709 (2017). https://doi.org/10.1007/s10517-017-3885-9
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-017-3885-9