Surface proteins of many bacterial species interact with human serum albumin (HSA) via a special region of amino acid sequence termed GA module. For instance, surface peptostreptococcal albumin-binding protein of anaerobic bacteria Peptostreptococcus magnus contains one HSA-binding GA-module. Protein G from group G and C Streptococcus strains isolated from humans has HSA-binding region consisting of three GA-modules. HSA-binding protein containing two GA-modules was found in strains of group G Streptococcus of animal origin. We obtained two recombinant polypeptides GA1 and GA2 congaing one GA-module each. Recombinant polypeptide with two GA-modules binds HSA with a much higher affinity than polypeptides GA1 and GA2 containing one GA-module. Polypeptide with the second GAmodule more effectively binds HSA than polypeptides with the GA-module.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 161, No. 7, pp. 79-83, July, 2016
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Bormotova, E.A., Gupalova, T.V. Production of Recombinant Polypeptides Containing One GA-Module and Analysis of Their Ability to Bind to Human Albumin. Bull Exp Biol Med 162, 66–70 (2016). https://doi.org/10.1007/s10517-016-3547-3
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DOI: https://doi.org/10.1007/s10517-016-3547-3