We studied the differences in twitch force of subendocardial and subepicardial cardiomyocytes isolated from mouse left ventricular wall at different preloads using an original single cell stretch method recently developed by us. Then, we used our mathematical models of subendocardial and subepicardial cells to predict underlying cellular mechanisms. Transmural differences in the amplitudes of active tension of subendocardial and subepicardial cardiomyocytes were revealed that could be related to the differences in cooperative end-to-end interaction between the neighboring regulatory units of the thin filament.
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Bryant SM, Shipsey SJ, Hart G. Regional differences in electrical and mechanical properties of myocytes from guinea-pig hearts with mild left ventricular hypertrophy. Cardiovasc. Res. 1997;35(2):315-323.
Cordeiro JM, Greene L, Heilmann C, Antzelevitch D, Antzelevitch C. Transmural heterogeneity of calcium activity and mechanical function in the canine left ventricle. Am. J. Physiol. Heart Circ. Physiol. 2004;286(4):H1471-H1479.
Iribe G, Kaneko T, Yamaguchi Y, Naruse K. Load dependency in force-length relations in isolated single cardiomyocytes. Prog. Biophys. Mol. Biol. 2014;115(2-3):103-114.
Khokhlova A, Iribe G, Solovyova O. Load-dependency in me chanical properties of subepicardial and subendocardial cardiomyocytes. CinC. 2015;42(1):965-968.
Stones R, Calaghan SC, Billeter R, Harrison SM, White E. Transmural variations in gene expression of stretch-mo dulated proteins in the rat left ventricle. Pflugers. Arch. 2007; 454(4):545-549.
Vasil’eva AD, Solov’eva OÉ. Electromechanical coupling in cardiomyocytes from transmural layers of guinea pig left ventricle. Biofizika. 2012;57(5):852-859.
Wan X, Bryant SM, Hart G. A topographical study of mechanical and electrical properties of single myocytes isolated from normal guinea-pig ventricular muscle. J. Anat. 2003; 202(6):525-536.
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Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 162, No. 7, pp. 58-61, July, 2016
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Khokhlova, A.D., Iribe, G. Transmural Differences in Mechanical Properties of Isolated Subendocardial and Subepicardial Cardiomyocytes. Bull Exp Biol Med 162, 48–50 (2016). https://doi.org/10.1007/s10517-016-3542-8
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DOI: https://doi.org/10.1007/s10517-016-3542-8