We studied the expression of an early activation marker CD69 in peripheral blood lymphocytes of pregnant women with a history of recurrent pregnancy loss after immunization with paternal lymphocytes. Spontaneous and phytohemagglutinin-stimulated expression of CD69 on the surface of T cells and NK cells isolated from the peripheral blood was analyzed. On gestation week 5-6, the number of T cells expressing CD69 spontaneously and after stimulation was significantly higher in women with miscarriage than in woman with prolonged pregnancy. However, the number of cells with CD56+ phenotype expressing CD69 did not differ in these groups. No differences were found in the number of cells of all subpopulations expressing CD69 after stimulation on gestation week 12 in woman with full-term current pregnancy and in woman with physiological pregnancy.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Krechetova LV, Stepanova EO, Nikolaeva MA, Vtorushina VV, Golubeva EL, Khachatryan NA, Tetruashvili NK, Sukhikh GT. Time course of changes in the peripheral blood lymphocyte subpopulation composition of women with recurrent miscarriage during pregravid immunocytotherapy. Akush. Ginekol. 2015:(5):33-38. Russian.
Pososeeva LV, Malushkina AI, Boiko EL, Sotnikova NYu, Peretyztko LP, Fetisova IN. Rehabilitation of reproductive health in couples with recurrent pregnancy loss. Ivanovo, 2008. Russian.
Sidel’nikova VM, Sukhikh GT. Recurrent Miscarriage. Moscow, 2011. Russian.
Coulam C.B, Roussev RG. Correlation of NK cell activation and inhibition markers with NK cytoxicity among women experiencing immunologic implantation failure after in vitro fertilization and embryo transfer. J. Assist. Reprod. Genet. 2003:20(2):58-62.
Ghafourian M, Karami N, Khodadadi A, Nikbakht R. Increase of CD69, CD161 and CD94 on NK cells in women with recurrent spontaneous abortion and in vitro fertilization failure. Iran J. Immunol. 2014:11(2):84-96.
Khonina NA, Broitman EV, Shevela EY, Pasman NM, Chernykh ER. Mixed lymphocyte reaction blocking factors (MLRBf) as potential biomarker for indication and efficacy of paternal lymphocyte immunization in recurrent spontaneous abortion. Arch. Gynecol. Obstet. 2013:288(4):933-937.
Nakashima A, Shima T, Inada K, Ito M, Saito S. The balance of the immune system between T cells and NK cells in miscarriage. Am. J. Reprod. Immunol. 2012:67(4):304-310.
Nonaka T, Takakuwa K, Ooki I, Akashi M, Yokoo T, Kikuchi A, Tanaka K. Results of immunotherapy for patients with unexplained primary recurrent abortions–prospective non-randomized cohort study. Am. J. Reprod. Immunol. 2007:58(6):530-536.
Quack KC, Vassiliadou N, Pudney J, Anderson DJ, Hill JA. Leukocyte activation in the decidua of chromosomally normal and abnormal fetuses from women with recurrent abortion. Hum. Reprod. 2001:16(5):949-955.
Ramhorst R, García V, Agriello E, Corigliano A, Etchepareborda E, Irigoyen M, Pasanante G, Fainboim L. Intracellular expression of CD69 in endometrial and peripheral T cells represents a useful marker in women with recurrent miscarriage: modulation after allogeneic leukocyte immunotherapy. Am. J. Reprod. Immunol. 2003:49(3):149-158.
Ramhorst RE, Fainboim L. New actors for immunological mechanisms involved in the materno-fetal tolerance. Current Women’s Health Rev. 2005:1(1):15-20. doi: 10.2174/1573404052950294.
Reccurent Pregnancy Loss: Causes, Controversies and Treatment. Howard JA Carp., ed. London, 2007.
Early Pregnancy, Farquharson RG, Stephenson MD, eds. Cambridge, 2012.
Vassiliadou N, Searle R.F, Bulmer JN. Elevated expression of activation molecules by decidual lymphocytes in women suffering spontaneous early pregnancy loss. Hum. Reprod. 1999:14(5):1194-1200.
Zabińska-Popiela M, Wicherek L, Gałazka K, Dutsch-Wicherek M, Kaim I, Popiela TJ, Krzysiek J, Basta A. Comparative analysis of CD56 lymphocytes and CD69 antigen expression in endometrium during decidualization. Przegl. Lek. 2006:63(4):176-178.
Author information
Authors and Affiliations
Corresponding author
Additional information
Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 161, No. 4, pp. 519-522, April, 2016
Rights and permissions
About this article
Cite this article
Krechetova, L.V., Vtorushina, V.V., Nikolaeva, M.A. et al. Expression of Early Activation Marker CD69 on Peripheral Blood Lymphocytes from Pregnant Women after First Trimester Alloimmunization. Bull Exp Biol Med 161, 529–532 (2016). https://doi.org/10.1007/s10517-016-3453-8
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10517-016-3453-8