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Isolation of Multipotent Mesenchymal Stromal Cells from Cryopreserved Human Umbilical Cord Tissue

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Bulletin of Experimental Biology and Medicine Aims and scope

Umbilical cord stroma is an easily available, convenient, and promising source of multipotent mesenchymal stromal cells for regenerative medicine. Cryogenic storage of umbilical cord tissue provides more possibilities for further isolation of multipotent mesenchymal stromal cells for autologous transplantation or scientific purposes. Here we developed a protocol for preparation of the whole umbilical cord tissue for cryogenic storage that in combination with the previously described modified method of isolation of multipotent mesenchymal stromal cells allowed us to isolate cells with high proliferative potential, typical phenotype, and preserved differentiation potencies.

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Authors and Affiliations

  1. Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia

    Yu. A. Romanov, E. E. Balashova & N. V. Kabaeva

  2. V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia

    E. E. Balashova, N. E. Volgina, T. N. Dugina & G. T. Sukhikh

  3. CryoCenter Umbilical Cord Blood Bank, Moscow, Russia

    Yu. A. Romanov

Authors
  1. Yu. A. Romanov
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  2. E. E. Balashova
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  3. N. E. Volgina
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  4. N. V. Kabaeva
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  5. T. N. Dugina
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  6. G. T. Sukhikh
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Corresponding author

Correspondence to Yu. A. Romanov.

Additional information

Translated from Kletochnye Tekhnologii v Biologii i Meditsine, No. 4, pp. 218-223, October, 2015

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Romanov, Y.A., Balashova, E.E., Volgina, N.E. et al. Isolation of Multipotent Mesenchymal Stromal Cells from Cryopreserved Human Umbilical Cord Tissue. Bull Exp Biol Med 160, 530–534 (2016). https://doi.org/10.1007/s10517-016-3213-9

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  • DOI: https://doi.org/10.1007/s10517-016-3213-9

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