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Comparative study on tetraploid induction of the Fujian oyster Crassostrea angulata utilizing three typical methods

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Abstract

The sterility advantages of triploids make them paramount for oyster aquaculture. Tetraploids are always used as male parents for triploid industrial breeding in current hatcheries, thus they are the core asset for triploid oyster culture. The oyster Crassostrea angulata is the most productive oyster species in China. However, the optimal conditions for tetraploid induction have never been examined. In this study, we investigated the effectiveness of three induction methods (Method I, 2n ♀ × 2n ♂ + inducer abbreviated as DD + ind; Method II, 3n ♀ × 2n ♂ + inducer abbreviated as TrD + ind; Method III, 2n ♀ × 4n ♂ + inducer abbreviated as DT + ind) and three inducers (salinity; cytochalasin B, CB; and 6-dimethylaminopurine, 6-DMAP) for tetraploid induction in C. angulata. The optimal induction conditions for the three induction methods were a CB concentration of 0.5 mg/L treated for 20 min, 0.75 mg/L CB for a constant induction treatment of 20 min and 6-DMAP at a concentration of 100 mg/L for 20 min, respectively. When the optimal induction conditions were applied to a single pair of oysters, the D-larval tetraploid rate for the three methods were 18.93 ± 1.46%, 58.32 ± 1.03% and 51.61 ± 1.37%, respectively. After 30 days, the survival rates of the experimental groups were only 10.36 ± 2.51%, 28.07 ± 2.03% and 21.99 ± 1.27%, respectively, at day 30. The tetraploid rates in DD + ind and DT + ind were both decreased significantly over time, but they still reached 11.81% and 21.99% at 30 days, and 8% and 16% at day 180, respectively. Tetraploid rates in TrD + ind were 58.32 − 71.28% at the larval stage and 80% at day 180. In summary, despite very different induction rates, surviving tetraploids of C. angulata could be obtained in all three methods. Method II (TrD + inducer) was regarded as the optimal method to induce C. angulata tetraploids, and the optimal induction condition was a CB concentration of 0.75 mg/L and treated for 20 min under this method.

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Data availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Funding

This work was supported by grants from the National Key R&D Program of China (2022YFD2400305), the China Agriculture Research System Project (CARS-49), and Shandong Province (2021ZLGX03, 2021LZGC027).

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Authors and Affiliations

  1. Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, 266003, China

    Yuanxin Liang, Geng Cheng, Xianchao Bai, Jianmin Zhou, Haining Zhang, Yong Chi, Gaowei Jiang, Chengxun Xu & Qi Li

  2. Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China

    Qi Li

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  1. Yuanxin Liang
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  2. Geng Cheng
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Contributions

Yuanxin Liang: Conceptualization, supervision, resources, project admi. Geng Cheng: Resources. Xianchao Bai: Resources. Jianmin Zhou: Conceptualization, supervision. Haining Zhang: Supervision. Yong Chi: Resources. Gaowei Jiang: Conceptualization. Chengxun Xu: Resources. Qi Li: Conceptualization, supervision, resources, project admi.All authors reviewed the manuscript.

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Correspondence to Qi Li.

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The present study was performed according to the standard operation procedures (SOPs) of the Guide for the Use of Experimental Animals of the Ocean University of China. All animal care and use procedures were approved by the Institutional Animal Care and Use Committee of Ocean University of China.

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Liang, Y., Cheng, G., Bai, X. et al. Comparative study on tetraploid induction of the Fujian oyster Crassostrea angulata utilizing three typical methods. Aquacult Int 32, 593–612 (2024). https://doi.org/10.1007/s10499-023-01174-9

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