Abstract
Parentage identification technology is of great significance in the selective breeding of aquatic animals. Procambarus clarkii, at present, is an important freshwater economic species in China, and its genetic breeding is quite urgent for its aquaculture. In this study, 18 novel polymorphic microsatellite markers were developed for P. clarkii from 144 potential primers. The efficiency of 16 developed tetrameric markers with higher PIC values and two published tetrameric markers for parentage identification was evaluated in 15 maternal half-sib families. Ten highly polymorphic markers (mean HE = 0.720 and PIC = 0.669) were identified as being suitable for inclusion in the parentage marker suite. Simulation analysis indicated that the cumulative exclusion probabilities for the ten loci were 97.55% and 99.86%, respectively, when no parental information or only one parental information was available. In actual parentage assignments, 94.67% of offspring were correctly assigned to their mothers, which confirmed the application value of this marker suite in the parentage identification of P. clarkii.
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Funding
We thank Guangdong HAID Group Co., Ltd. for providing some genotyping cost support in this study. This work was supported by the Fundamental Research Funds for the Central Universities (2662020SCPY004) and the National Key Research and Development Program of China (2020YFD0900304).
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YL conceived and designed the experiments. QW, QH, and SY performed the experiments. QW analyzed the data and drafted the manuscript. YL revised the manuscript. All authors have read and approved the final manuscript.
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This study has been approved by the Institutional Animal Care and Use Committee (IACUC) of Huazhong Agricultural University (Wuhan, China) and conducted in accordance with ethical standards and according to the national and international guidelines.
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Wang, Q., Hu, Q., Yang, S. et al. Isolation of tetrameric microsatellite markers and its application on parentage identification in Procambarus clarkii. Aquacult Int 31, 2099–2111 (2023). https://doi.org/10.1007/s10499-023-01072-0
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DOI: https://doi.org/10.1007/s10499-023-01072-0