Abstract
The cultivation of Chlorella vulgaris in sludge extract (SE) from municipal excess sludge diluted with BG11 medium was investigated to achieve sludge reduction and microalgae cultivation. A maximum biomass of 1.404 ± 0.101 g/L was achieved at 15 days in a mixture of 25% BG11 and 75% sludge extract under 10,000-lx light. In early culture periods, heterotrophy dominated the process in the group with a high proportion of sludge extract of excess sludge extract containing relatively high amounts of organic material, which led to enhanced citrate synthase enzyme activities and reduced rubisco enzyme activities. Moreover, increasing the light intensity did not significantly improve biomass production due to its inhibitory effects on respiration in the early culture stages. The response surface model showed that organic matter was the main factor influencing biomass production from 0 to 2 days. The influence of light intensity on biomass production became more noticeable only after depleting the organic matter in sludge extract.
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References
Åkerström AM, Mortensen LM, Rusten B, Gislerød HR (2014) Biomass production and nutrient removal by Chlorella sp. as affected by sludge liquor concentration. J Environ Manag 144:118–124. https://doi.org/10.1016/j.jenvman.2014.05.015
Amini KZ, Seyfabadi J, Ramezanpour Z (2012) Effect of light intensity and photoperiod on biomass and fatty acid composition of the microalgae, Chlorella vulgaris. Aquac Int 20:41–49. https://doi.org/10.1007/s10499-011-9440-1
Angerbauer C, Siebenhofer M, Mittelbach M, Guebitz GM (2008) Conversion of sewage sludge into lipids by Lipomyces starkeyi for biodiesel production. Bioresour Technol 99(8):3051–3056. https://doi.org/10.1016/j.biortech.2007.06.045
Baker NR (2020) Chlorophyll fluorescence: a probe of photosynthesis in vivo. Annu Rev Plant Biol 59:89–113. https://doi.org/10.1146/annurev.arplant.59.032607.092759
Chen X, Wei X, Wang J, Yang Y, Wang Y, Li Q, Wang S (2020) Toxicity removal and biodegradability enhancement of sludge extract in hydroquinone-rich wastewater via cultivation of Chlorella vulgaris. J Clean Prod 277:124030. https://doi.org/10.1016/j.jclepro.2020.124030
Christe M, Hirzel E, Lindinger A et al (2014) Obesity affects mitochondrial citrate synthase in human omental adipose tissue. Isrn Obesity 2013(3, part 2):826027. https://doi.org/10.13039/100010381
Endo H, Sansawa H, Nakajima K (1977) Studies on Chlorella regularis, heterotrophic fast-growing strain II. Mixotrophic growth in relation to light intensity and acetate concentration. Plant Cell Physiol 18:199–205. https://doi.org/10.1093/oxfordjournals.pcp.a075413
Hadjromdhane F, Zheng X, Jaouen P, Pruvost J, Grizeau D, Croue JP, Bourseau P (2013) The culture of Chlorella vulgaris in a recycled supernatant: effects on biomass production and medium quality. Bioresour Technol 132:285–292. https://doi.org/10.1016/j.biortech.2013.01.025
Kim S, Park JE, Cho YB, Hwang SJ (2013) Growth rate, organic carbon and nutrient removal rates of Chlorella sorokiniana, in autotrophic, heterotrophic and mixotrophic conditions. Bioresour Technol 144:8–13. https://doi.org/10.1016/j.biortech.2013.06.068
Lan Y. Mott K A (1991) Determination of apparent Km values for ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) activase using the spectrophotometric assay of Rubisco activity. Plant Physiol 95: 604-609. https://doi.org/10.1104/pp.95.2.604
Lay JJ, Fan kS, Ku CH (2003) Influence of chemical nature of organic wastes on their conversion to hydrogen by heat-shock digested sludge. Int J Hydrogen Energ 28:1361–1367. https://doi.org/10.1016/S0360-3199(03)00027-2
Li T, Kirchhoff H, Gargouri M, Feng J, Cousins AB, Pienkos PT, Gang D, Chen S (2016) Assessment of photosynthesis regulation in mixotrophically cultured microalga Chlorella sorokiniana. Algal Res 19:30–38. https://doi.org/10.1016/j.algal.2016.07.012
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ, Lewis A (1951) Protein measurement with the folin. J boil chem 193:265–275. https://doi.org/10.1515/bchm2.1951.286.1-6.270
Osundeko O, Pittman JK (2014) Implications of sludge liquor addition for wastewater-based open pond cultivation of microalgae for biofuel generation and pollutant remediation. Bioresour Technol 152:355–363. https://doi.org/10.1016/j.biortech.2013.11.035
Pearsall WH, Bengry RP (1940) Growth of Chlorella in relation to light intensity. Ann Bot 4:485–494
Racker E (1962) Ribulose diphosphate carboxylase from spinach leaves. Methods Enzymol 5:266–270. https://doi.org/10.1016/0076-6879(75)42153-X
Rym BD, Nejeh G, Lamia T, Ali Y, Rafika C, Khemissa G (2010) Modeling growth and photosynthetic response in Arthrospira platensis, as function of light intensity and glucose concentration using factorial design. J Appl Phycol 22(6):745–752. https://doi.org/10.1007/s10811-010-9515-9
Shepherd D, Garland PB (1969) The kinetic properties of citrate synthase from rat liver mitochondria. Biochem J 114(3):597–610. https://doi.org/10.1042/bj1140597
Uggetti E, Ferrer I, Llorens E, García J (2010) Sludge treatment wetlands: a review on the state of the art. Bioresour Technol 101:2905–2912. https://doi.org/10.1016/j.biortech.2009.11.102
Wang L, Wang H, Chen X, Xu Y, Zhou T, Wang X, Lu Q, Ruan R (2018) Using Chlorella vulgaris to treat toxic excess sludge extract, and identification of its response mechanism by proteomics approach. Bioresour Technol 253:188–196. https://doi.org/10.1016/j.biortech.2018.01.039
Yan R, Zhu D, Zeng Q, Chu J (2012) Carbon metabolism and energy conversion of Synechococcus sp. pcc 7942 under mixotrophic conditions: comparison with photoautotrophic condition. J Appl Phycol 24:657–668. https://doi.org/10.1007/s10811-011-9683-2
Zhang Z, Sun D, Wu T, Li Y, Lee YK, Liu YJ, Chen F (2017) The synergistic energy and carbon metabolism under mixotrophic cultivation reveals the coordination between photosynthesis and aerobic respiration in Chlorella zofingiensis. Algal Res 252:109–116. https://doi.org/10.1016/j.algal.2017.05.007
Zuliani L, Frison N, Jelic A, Fatone F, Bolzon D, Ballottari M (2016) Microalgae cultivation on anaerobic digestate of municipal wastewater, sewage sludge and agro-waste. Int J Mol Sci 17:1692. https://doi.org/10.3390/ijms17101692
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This research was supported by the National Key Research and Development Program of China (2017YFB0602804), the Major Projects of Science and Technology (no. 2014ZX07202-011) in China, the National Natural Science Foundation (no. 51378207&51878278), and the Shanghai Pujiang Programme (no. 13PJD009), the Fundamental Research Funds for the Central Universities (222201817009).
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Chen, X., Yang, Y., Lu, Q. et al. The influence of light intensity and organic content on cultivation of Chlorella vulgaris in sludge extracts diluted with BG11. Aquacult Int 29, 2131–2144 (2021). https://doi.org/10.1007/s10499-021-00740-3
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DOI: https://doi.org/10.1007/s10499-021-00740-3