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Functional and bioactive properties of chitosan from Indian major carp scale

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Abstract

Chitin is the most important natural polysaccharide after cellulose; however, it is not widely used for industrial application because of its insolubility in many solvents. The present study utilizes Indian major carps (Labeo rohita, Catla catla, and Cirrhinus mrigala) scale to extract chitin and prepare chitosan by a sequence of chemical processes involving demineralization, deproteinization, and deacetylation. The extracted chitosan is characterized by different analytical techniques viz. UV-Vis spectroscopy, FT-IR, TGA, and XRD, and SEM analysis reveals that fish scale waste are good source of chitosan. Compositional analysis of fish scale waste was done by standard methods which reveal that the extracted chitosan was having 0.691% moisture content, 0.53% ash, 109% fat-binding assay (FBC), 104% water-binding assay (WBC) indicating the value depend upon the source of origin and optimization of chemical process of extraction. The antibacterial activity of chitosan against Aeromonas hydrophila, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa was evaluated by agar well diffusion method. The chitosan also exhibited DPPH scavenging along with anti-inflammatory and anticoagulant activity in a dose-dependent manner. The extracted chitosan also demonstrated preservation and seed germination activities. The result of the present study strongly supports the use of chitosan in pharmaceutical, food industry, and agro-based industries.

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References

  • Allison PG, Rodriguez RI, Moser RD, Williams BA, Poda AR, Seiter JM, Chandler MQ (2014) Characterization of multi-layered fish scales (Atractosteus spatula) using nanoindentation, X-ray CT, FTIR, and SEM. J Vis Exp 89:e51535

    Google Scholar 

  • Al-Tawaha ARM, Al-Ghzawi ALA (2013) Effect of chitosan coating on seed germination and salt tolerance of lentil (Lens culinaris L.). Res Crops 14:489–491

    Google Scholar 

  • Andrade S, Ladchumananandasivam R, Rocha B, Belarmino D, Galvao A (2012) The use of exoskeletons of shrimp (Litopenaeus vanammei) and crab (Ucides cordatus) for the extraction of chitosan and production of nanomembrane. Enzyme Microb Tech 3:495–508

    Google Scholar 

  • Chung YC, Kuo CL, Chen CC (2005) Preparation and important functional properties of water-soluble chitosan produced through Maillard reaction. Bioresour Technol 96:1473–1482

    Article  CAS  Google Scholar 

  • Das SK, Samantaray D, Mahapatra A, Pal N, Munda R, Thatoi HN (2018) Pharmacological activities of leaf and bark extracts of a medicinal mangrove plant Avicennia officinalis L. Clin Phytosci 4:13. https://doi.org/10.1186/s40816-018-0072-0

    Article  CAS  Google Scholar 

  • Di Martino A, Sittinger M, Risbud MV (2005) Chitosan: a versatile biopolymer for orthopaedic tissue engineering. Biomaterials 26:5983–5990

    Article  Google Scholar 

  • Gokulalakshmi E, Ramalingam K, Umasankari VMC (2017) Extraction and characterization of chitosan obtained from scales of Clarias gariepinus (catfish). Biotechnol J Int 18:1–8

    Article  Google Scholar 

  • Helrich K (1990) Official methods of analysis of the association of official analytical chemists. 15th ed. Virginia, USA

  • Islam MS, Khan S, Tanaka M (2004) Waste loading in shrimp and fish processing effluents: potential source of hazards to the coastal and near shore environments. Mar Pollut Bull 49:103–110

    Article  CAS  Google Scholar 

  • Jeyaraman M, Vignesh R, Sumathi N (2017) Extraction, characterization and applications of chitosan from fish scales. Int J Mod Trends Eng Res 4:137–141

    Google Scholar 

  • Kaya M, Seyyar O, Baran T, Türkeş T (2014) Bat guano as new and attractive chitin and chitosan source. Front Zool 11:59. https://doi.org/10.1186/s12983-014-0059-8

    Article  CAS  Google Scholar 

  • Knorr D (1982) Functional properties of chitin and chitosan. J Food Sci 47:593–595

    Article  CAS  Google Scholar 

  • Krajewska B (2004) Application of chitin- and chitosan-based materials for enzyme immobilizations: a review. Enzyme Microb Technol 35:126–139

    Article  CAS  Google Scholar 

  • Kumari S, Rath P (2014) Extraction and characterization of chitin and chitosan from (Labeo rohita) fish scales. Procedia Mater Sci 6:482–489

    Article  CAS  Google Scholar 

  • Kumari S, Rath P, Kumar ASH (2016) Chitosan from shrimp shell (Crangon crangon) and fish scales (Labeo rohita): extraction and characterization. Afr J Biotechnol 15:1258–1268

    Article  CAS  Google Scholar 

  • Li R, He J, Xie H, Wang W, Bose SK, Sun Y, Hu J, Yin H (2019) Effects of chitosan nanoparticles on seed germination and seedling growth of wheat (Triticum aestivum L.). Int J Biol Macromol 126:91–100

    Article  CAS  Google Scholar 

  • Mohanasrinivasan V, Mishra M, Paliwal JS, Singh K, Selvarajan E, Suganthi V, Devi CS (2013) Studies on heavy metal removal efficiency and antibacterial activity of chitosan prepared from shrimp shell waste. 3 Biotech 4:167–175

    Article  Google Scholar 

  • Murugan R, Parimelazhagan T (2014) Comparative evaluation of different extraction methods for antioxidant and anti-inflammatory properties from Osbeckiaparvi folia Arn.–an in vitro approach. J King Saud Univ Sci 26:267–275

    Article  Google Scholar 

  • Nising P (2006) High-temperature radical polymerization of methyl methacrylate in a continuous pilot scale process [Ph.D. Thesis]. Ecole Polytechnique Federale De Lausanne Lausanne: EPFL

  • No HK, Meyers SP (1992) Utilization of crawfish processing waste as carotenoids, chitin and chitosan sources. J Korean Soc Food Nutr 21:319–326

    CAS  Google Scholar 

  • Panith N, Wichaphon J, Lertsiri S, Niamsiri N (2016) Effect of physical and physicochemical characteristics of chitosan on fat-binding capacities under in vitro gastrointestinal conditions. LWT-Food Sci Technol 71:25–32

    Article  CAS  Google Scholar 

  • Paul S, Jayan A, Sasikumar CS, Cherian SM (2014) Extraction and purification of chitosan from chitin isolated from sea prawn Fenneropenaeus indicus. Asian J Pharm Clin Res 7:201–204

    Google Scholar 

  • Qin C, Li H, Xiao Q, Liu Y, Zhu J, Du Y (2006) Water-solubility of chitosan and its antimicrobial activity. Carbohydr Polym 63:367–374

    Article  CAS  Google Scholar 

  • Rajalakshmi A, Narayanaswamy K, Jayachitra A (2013) Antioxidant activity of the chitosan extracted from shrimp exoskeleton. Middle East J Sci 16:1446–1451

    CAS  Google Scholar 

  • Suman TY, Rajasree SR, Ramkumar R, Rajthilak C, Perumal P (2013) The green synthesis of gold nanoparticles using an aqueous root extract of Morinda citrifolia L. Spectrochim Acta A 118C:11–16

    Google Scholar 

  • Tarafdar A, Biswas G (2013) Extraction of chitosan from prawn shell wastes and examination of its viable commercial applications. Int J Theor Appl Res Mech Engg 2:2319–3182

    Google Scholar 

  • Trung TS, Bao HND (2015) Physicochemical Properties and antioxidant activity of chitin and chitosan prepared from pacific white shrimp waste. Int J Carbohydr Chem, pp 1–6. https://doi.org/10.1155/2015/706259

  • Walke S, Srivastava GK, Nikalje MD, Doshi JB, Kumar R, Ravetkar SD, Doshi PJ (2014) Physicochemical and functional characterization of chitosan prepared from shrimp shells and investigation of its antibacterial, antioxidant and tetanus toxoid entrapment efficiency. Int J Pharm Sci Rev Res 26:215–225

    CAS  Google Scholar 

  • Wang JC, Kinsella JE (1976) Functional properties of novel proteins: alfalfa leaf protein. J Food Sci 41:286–292

    Article  CAS  Google Scholar 

  • Zaku SG, Emmanuel SA, Aguzue OC, Thomas SA (2011) Extraction and characterization of chitin; a functional biopolymer obtained from scales of common carp fish (Cyprinus carpio l.): a lesser known source. Afr J Food Sci 5(8):478–483

    CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Biotechnology, College of Engineering and Technology, Biju Patnaik University of Technology, Bhubaneswar, Odisha, 751029, India

    Ark Ansuman Satpathy, Supriya Dash, Swagat Kumar Das & Shyamalini Shyamasuta

  2. Department of Chemistry, College of Engineering and Technology, Biju Patnaik University of Technology, Bhubaneswar, Odisha, 751029, India

    Suvendu Pradhan

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  1. Ark Ansuman Satpathy
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  2. Supriya Dash
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  3. Swagat Kumar Das
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  4. Shyamalini Shyamasuta
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  5. Suvendu Pradhan
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Correspondence to Supriya Dash.

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Satpathy, A.A., Dash, S., Das, S.K. et al. Functional and bioactive properties of chitosan from Indian major carp scale. Aquacult Int 29, 417–430 (2021). https://doi.org/10.1007/s10499-020-00622-0

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