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Correlation between C-reactive protein level, immunology, and hematology of a Oncorhynchus mykiss infected with Lactococcus garvieae

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Abstract

The aim of this experiment was to evaluate the symptoms, variation, and correlation of hematological factors and some parameters of the innate immune response of the infected rainbow trout (Oncorhynchus mykiss) fish with Lactococcus garvieae bacteria. A total of 270 fish were divided into three groups including infected fish with a high and low concentration of L. garvieae and a control group without infection. The blood and tissue (brain, head, kidney, and spleen) samples were collected from each group (n = 6 fish) at 0, 3, 14, and 21 days after treatment. The most observed symptoms during infection were lethargy, exophthalmia, and ascites. The mortality rates for the high and low dose-infected fish were defined as 60 and 25%, respectively. The infected groups had significantly (p < 0.05) higher activity of serum lysozyme, myeloperoxidase, classical and alternative pathways of complement, serum bactericidal effects, and the specific antibody titer. Also, a significant (p < 0.05) lower hematocrit and hemoglobin levels and higher white blood cell numbers were observed in the infected groups. A significant correlation was observed between the CRP levels and some of the hematological and immunological indices as bactericidal effects, classical complement pathways, lysozyme activity myeloperoxidase activity, white blood cell numbers, and hematocrit levels. The clinical symptoms, immune responses, and hematological indices variation among L. garvieae-infected fish are dependent on the duration and bacterial dose of the infection.

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Funding

This experiment was financially supported by the Shahid Chamran University of Ahvaz grant.

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Authors and Affiliations

  1. Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

    Mohammad Khosravi

  2. Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

    Takavar Mohammadian

  3. Graduate Student of Veterinary Medicine, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

    Mina Tahmasebifard

  4. Department of Basic science, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran

    Mehdi Pourmehdi Boroujeni

Authors
  1. Mohammad Khosravi
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  2. Takavar Mohammadian
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  3. Mina Tahmasebifard
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  4. Mehdi Pourmehdi Boroujeni
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Corresponding author

Correspondence to Mohammad Khosravi.

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Ethics approval and consent to participate

All experimental protocols were approved by the research program animal care and use committee of the Shahid Chamran University of Ahvaz, Iran (905824/28-10-2017).

Conflict of interest

The authors declare that they have no conflict of interest.

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Khosravi, M., Mohammadian, T., Tahmasebifard, M. et al. Correlation between C-reactive protein level, immunology, and hematology of a Oncorhynchus mykiss infected with Lactococcus garvieae. Aquacult Int 26, 1415–1425 (2018). https://doi.org/10.1007/s10499-018-0295-6

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  • DOI: https://doi.org/10.1007/s10499-018-0295-6

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