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Protein requirement of juvenile striped catfish, Pangasianodon hypophthalmus

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Abstract

A trial was conducted to determine the dietary protein requirement for growth of juvenile striped catfish (Pangasianodon hypophthalmus). Iso-energetic semi-purified diets (~ 19.78 MJ kg−1) with six levels of protein 20% (T1), 25% (T2), 30% (T3), 35% (T4), 40% (T5), and 45% (T6) were fed to triplicate groups of fish (initial mean weight 4.27 to 4.57 g) to satiation twice daily for 60 days. Growth, feed conversion, protein utilization, intestinal digestive enzyme activities, and body composition were measured as response variables. Best growth (P < 0.05) was observed in fish fed with 40% dietary protein, and second order polynomial regression analysis (y = − 0.0011x2 + 0.0816x + 0.0642; R 2 = 0.9241; dietary protein levels as independent variable and specific growth rate as dependent variable) gave a protein requirement for growth of 37.1%. A minimum feed conversion ratio (FCR = 1.38) was found in fish fed with 35% dietary protein. Apparent net protein utilization (ANPU) was found to decrease (from 39.57 to 16.15%), with increasing dietary protein levels (P < 0.05). Protease and amylase activities were found to be in opposition to each other, with increasing dietary protein levels. The highest protease (0.59 μmol of tyrosine released min−1 mg−1 protein) and lowest amylase activities (7.99 μmol of maltose released min−1 mg−1 protein) were observed in fish fed with 40% dietary protein (P < 0.05); whereas lipase activity (P > 0.05) was found to be independent of dietary protein levels. Whole body carcass composition (except ether extract) varied with the dietary protein level, and significant higher values of moisture content (72.31%), crude protein (18.83%), and ash content (4.33%) were observed in the treatment group fed with 40% dietary protein. Hence, it may be concluded that the dietary protein requirement for growth of P. hypophthalmus juveniles is 37.1%.

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Abbreviations

ANPU:

Apparent net protein utilization

°C:

Degree centigrade

cm:

Centimeters

FCR:

Feed conversion ratio

HSI:

Hepatosomatic index

kg:

Kilogram

g:

Gram

hrs:

Hours

L:

Liter

M:

Molar

mg:

Milligram

MJ:

Megajoule

ml:

Milliliter

mm:

Millimeter

min:

Minutes

nm:

Nanometer

psi:

Pound-force per square inch

rpm:

Revolutions per minute

SGR:

Specific growth rate

VSI:

Viscerosomatic index

μL:

Microliter

%:

Percent

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Acknowledgements

We are thankful to the Director of the ICAR-Central Institute of Fisheries Education, Mumbai for providing well-equipped laboratories for the research work and the Indian Council of Agricultural Research, New Delhi for financial support. The authors also acknowledge Mary J. Nickum for English editing of the manuscript.

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Authors and Affiliations

  1. Fish Nutrition, Biochemistry and Physiology Division, ICAR-Central Institute of Fisheries Education, Mumbai, 400061, India

    Manish Jayant, Ande Penchala Muralidhar, Narrottam Prasad Sahu, Kamal Kant Jain, Asim Kumar Pal & Prem Prakash Srivastava

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  1. Manish Jayant
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  2. Ande Penchala Muralidhar
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  3. Narrottam Prasad Sahu
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  4. Kamal Kant Jain
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  5. Asim Kumar Pal
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  6. Prem Prakash Srivastava
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Correspondence to Manish Jayant.

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Jayant, M., Muralidhar, A.P., Sahu, N.P. et al. Protein requirement of juvenile striped catfish, Pangasianodon hypophthalmus . Aquacult Int 26, 375–389 (2018). https://doi.org/10.1007/s10499-017-0216-0

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