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Effect of temperature and nitrogen concentration on the growth and lipid content of Spirulina platensis and biodiesel production

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Abstract

In this study, Spirulina platensis (Cyanophyceae) was cultured at five mediums with different amounts of sodium nitrate and three different temperatures. The study was conducted at 1600 lx light intensity and a pH value of 9. The influences of nitrate amounts and temperature on biomass growth and lipid amount were studied during 20 days. The best biomass growth (1.039 ± 0.668 g L−1 dry weight and 0.780 ± 0.501 optical density) for S. platensis was obtained from the group with the temperature value of 35 °C and 2.5 g L−1 of sodium nitrate. The highest lipid production (4.70 ± 0.067% dry weight) was in the nitrate-free medium at 15 °C. The best results for biomass growth and lipid production were obtained from the group at 35 °C and 1.25 g L−1 of sodium nitrate (P < 0.05). At the end of the study, it was determined that algal biomass increased with increasing the nitrate amount but the produced lipid by the algae decreased. Intensive production was performed in accordance with the culture conditions of the most productive groups and biodiesel was obtained from the algae. The density, viscosity, and flash point of the biodiesel were calculated as 0.78, 4.67, and 107 °C, respectively.

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References

  • Antolin G, Tinaut FV, Briceno Y, Castano V, Perez C, Ramirez AI (2002) Optimisation of biodiesel production by sunflower oil transesterification. Bioresour Technol 83:111–114

    Article  CAS  PubMed  Google Scholar 

  • Belay A, Kato T, Ota Y (1996) Spirulina (Arthrospira): potential application as an animal feed supplement. JApplPhycol 8:303–311

    Google Scholar 

  • Baunillo KE, Tan RS, Barros HR, Luque R (2012) Investigations on microalgal oil production from Arthrospira platensis: towards more sustainable biodiesel production. RSC Adv 2:11267–11272

    Article  CAS  Google Scholar 

  • Chen F, Zhang Y, Guo S (1996) Growth and phycocyanin formation of Spirulina platensis in photoheterotrophic culture. Biotechnol Lett 18:603–608

    Article  CAS  Google Scholar 

  • Ciferri O (1983) Spirulina, the edible microorganism. Microbiol Rev 47:551–578

    CAS  PubMed  PubMed Central  Google Scholar 

  • Conti J.J, Holtberg P.D, Beaman, J.A, Schaal A.M, Sweetman G.E, Kydes A.A (2009) Annual Energy Outlook 2009. Energy information administration official energy statitics from the U.S. Goverment

  • Costa J, Cozza K, Oliveira L, Magagnin G (2001) Different nitrogen sources and growth responses of Spirulina platensis in microenvironments. World J Microbiol Biotechnol 17:439–442

    Article  CAS  Google Scholar 

  • Danesi E.D.G, Rangel C.O, Pelizer L.H, Carvalho J.C.M, Sato S, Moraes I.O. (2001) Production of Spirulina platensis under different temperatures and urea feeding regimes for chlorophyll attainment. In: Proceedings of the Eighth International Congress on Engineering and Food. 2: 1978–1982

  • Felizardo P, Correia MJN, Raposo I, Mendes JF, Berkemeier R, Bordado JM (2006) Production of biodiesel from waste frying oil. Waste Manag 26(5):487–494

    Article  CAS  PubMed  Google Scholar 

  • Fox D (1983) Algaculture, The microlagae Spirulina (Cyanophyceae). A study of the conditions necessary for their growth, These de Doctorat, France

  • Fukoda H, Kondo A, Noda H (2001) Biodiesel fuel production by transesterification of oils. J Biosci Bioeng 92:405–416

    Article  Google Scholar 

  • Glazer A (1999) Phycobiliproteins. In: Cohen Z (ed) Chemicals from microalgae. Taylor and Franchis Ltd., Philadelphia, PA, pp 261–276

    Google Scholar 

  • Iwata K (1990) Effects of spirulina on plasma lipoprotein lipase activity in rats. Pub in Journal Nutr Sci Vitaminol 36:165–171

    Article  CAS  Google Scholar 

  • Knothe G, Dunn R.O., Bagby M. O. (1997) Biodiesel: The use of vegetable oils and their derivaties as alternative diesel fuels. http://www.biodiesel.org/resources/reportdatabase/reports/gen/19961201_gen-162.pdf National Biodiesel Board. USA.

  • Lang X, Dalai AK, Bakhshi NN, Reaney MJ, Hertz PB (2001) Preparation and characterization of bio-diesels from various bio-oils. Bioresour Technol 80:53–62

    Article  CAS  PubMed  Google Scholar 

  • Lee SL, Yoon BD, Oh HM (1998) Rapid method for the determination of lipid from the green alga Botryococcus braunii. Biotechnology Techniques, Vol 12:553–556

    Article  CAS  Google Scholar 

  • Li Y, Horsman M, Wang B, Wu N, Lan CQ (2008) Effects of nitrogen sources on cell growth and lipid accumulation of green alga Neochloris oleoabundans. Appl Microbiol Biotechnol 81:629–636

    Article  CAS  PubMed  Google Scholar 

  • Miao X, Wu Q (2006) Biodiesel production from heterotrophic microalgal oil. Bioresour Technol 97:841–884

    Article  CAS  PubMed  Google Scholar 

  • Monbiot G. (2003) Bottom of the barrel. The Guardian, December 2, 2003. http://www.guardian.co.uk/politics/2003/dec/02/oil.greenpolitics

  • Richmond A. (1988) Spirulina. In: Micro-algal Biotechnology. Cambridge University Press, Cambridge. 85–121

  • Sayre R (2010) Microalgae: the potential for carbon capture. Bioscience 60:722–727

    Article  Google Scholar 

  • Seyhaneyıldız Can Ş, Demir V, Can E (2015) Evaluating the dilution of municipal wastewater on biomas increase, lipid production and nutrient removal by the blue-green algae Spirulina platensis (Geitler). Fresenius Environ Bull 24(3a):904–909

    Google Scholar 

  • Tanticharoen M, Reungjitchachawali M, Boonag B, Vonktaveesuk P, Vonshak A, Cohen Z (1994) Optimization of gamma-linolenic acid (GLA) production in Spirulina platensis. J Appl Phycol 6:295–300

    Article  CAS  Google Scholar 

  • Tedesco MA, Duerr EO (1989) Light, temperature and nitrogen starvation effects on the total lipid and fatty acid content and composition of Spirulina platensis UTEX 1928. J Appl Phycol 1:201–209

    Article  Google Scholar 

  • Wikdors GH, Ohno M (2001) Impact of algal research in aquaculture. JPhycol 37:968–974

    Google Scholar 

  • Zhang C (1994) Effects of polysaccharide and phycocyanin from Spirulina on peripheral blood and hematopoietic system of bone marrow in mice. Nanjing Univ

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Authors and Affiliations

  1. Engineering Faculty, Bioengineering Department, Munzur University, 62000, Tunceli, Turkey

    Safak Seyhaneyildiz Can

  2. Fisheries Faculty, Aquaculture Department, Ege University, 35100, İzmir, Turkey

    Edis Koru & Semra Cirik

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  1. Safak Seyhaneyildiz Can
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  2. Edis Koru
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  3. Semra Cirik
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Correspondence to Safak Seyhaneyildiz Can.

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Seyhaneyildiz Can, S., Koru, E. & Cirik, S. Effect of temperature and nitrogen concentration on the growth and lipid content of Spirulina platensis and biodiesel production. Aquacult Int 25, 1485–1493 (2017). https://doi.org/10.1007/s10499-017-0121-6

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  • DOI: https://doi.org/10.1007/s10499-017-0121-6

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