Abstract
Mites are among the major sources of domestic and occupational allergens worldwide, and continuous exposure to these allergens leads to chronic airway inflammation. One of the most allergenic species is the storage mite Tyrophagus putrescentiae (Schrank). Protein extracts are produced from this mite for tests that help the clinical diagnosis (via prick test), treatment, and monitoring of disease progression in patients who had positive results for allergic reactions. Therefore, the aim of the present study was to evaluate the cell viability of RAW 264.7 and L929 cells when exposed to in-house raw protein extracts of T. putrescentiae compared to a commercial product, as well as quantify TNF-α secretion by RAW 264.7. Additionally, this study quantified the effect of these extracts in IgE secretion in total blood of people affected by this mite. The study found similarity between the in-house extract and the commercial extract as they had equivalent TNF-α secretion. Additionally, viabilities of RAW 264.7 and L929 exposed to the in-house extract were compatible with viabilities of cells exposed to the commercial extract, with no cytotoxicity at the concentrations tested. Results corroborated the hypothesis that the extract produced in-house would be equivalent to the commercial extract in allergic patients when the IgE was quantified. This study is the first to show the cytotoxicity of T. putrescentiae extracts, and to provide a quantitative analysis of TNF-α and IgE.
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03 August 2023
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Acknowledgements
The authors are grateful to Universidade do Vale do Taquari - Univates and FAPERGS (21/2551-0000083-0) for their financial support, scholarships and for providing the material required to conduct this study. We thank Mrs. Daniela Tannus for improving the English of the manuscript.
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This work was supported by the FAPERGS under Grant 21/2551-0000083-0.
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Damo, L.H., Ribeiro, L.A.N., Vieira, L. et al. Effects of in-house and commercial extracts of the allergenic mite Tyrophagus putrescentiae on murine and human cell responses. Exp Appl Acarol 90, 33–45 (2023). https://doi.org/10.1007/s10493-023-00801-7
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DOI: https://doi.org/10.1007/s10493-023-00801-7