Abstract
Generally, males increase their reproductive success by mating with as many females as possible, whereas females increase their reproductive success by choosing males who provide more direct and indirect benefits. The difference in reproductive strategy between the sexes creates intense competition among males for access to females, therefore males spend much energy and time for competition with rival males for their reproduction. However, if they do not need to engage themselves into male competition and females are in no short supply, how many females can a male mate with and fertilize? We address this question in the two-spotted spider mite, Tetranychus urticae Koch. In this study, we investigated how many females a young, virgin male mated in 3 h, and checked whether the mated females were fertilized. We found that on average males mated with 12–13 females (range: 5–25). As latency to next mating did not change with the number of matings, the males are predicted to engage in even more matings if the mating trial were continued beyond 3 h. Copulation durations decreased with the number of matings and typically after 11 copulations with females any further copulations did not lead to fertilization, suggesting that males continued to mate with females even after sperm depletion. We discuss why spider mite males continue to display mating and copulation behaviour even after their sperm is depleted.
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Acknowledgements
We thank students who joined the practice courses in 2020: ‘Laboratory and field studies in biodiversity of model organism’ and ‘Advanced field course in the ecology of selected model organisms’, Ms. Yukine Kawamura, Ms. Nanako Yasuhara, Ms. Misaki Ono, Mr. Yakumo Watanabe and Mr. Takunori Yasuda in University of Tsukuba for their help in mating behaviour observation in T. urticae. We thank Mr. Gomei Yoda, Mr. Naoki Matsumoto and Mr. Shota Konaka for their help in the research project. We also thank Drs. Yosuke Degawa, Natsumi Kanzaki and Yoshiaki Iwamoto for their cooperation in the research project. This research was supported in part by JSPS KAKENHI Grant Number 20K06810 (Grant-in-Aid for Scientific Research C to YS).
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YS and HK contributed to the study conception and design. Material preparation, data collection and analysis were performed by HK and YS. The first draft of the manuscript was written by YS and ME and all authors read and approved the final manuscript.
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Supplementary file1 (TIFF 879 kb)
Figure S1 Copulation duration (a) and latency to mate (b) along sequential number of matings (mating order) in each male of Tetranychus urticae. Results from the same male are connected by grey dotted lines along time (h). Bold lines and filled areas along the lines in (b) indicate the predicted copulation durations and the 95% confidence interval by the models for series A and B in Table 2b, c. In (a) no line is shown because mating order did not have a significant effect on latency to mate
Supplementary file2 (TIFF 879 kb)
Figure S1 Copulation duration (a) and latency to mate (b) along sequential number of matings (mating order) in each male of Tetranychus urticae. Results from the same male are connected by grey dotted lines along time (h). Bold lines and filled areas along the lines in (b) indicate the predicted copulation durations and the 95% confidence interval by the models for series A and B in Table 2b, c. In (a) no line is shown because mating order did not have a significant effect on latency to mate
Supplementary file3 (TIFF 879 kb)
Figure S2 Female ratio in offspring along sequential number of matings (mating order) in each male of Tetranychus urticae. Results from the same male are connected by grey dotted lines along time (h). The bold solid line and filled area along the line indicate the predicted female fertilization status and the 95% confidence interval by the model in Table 4c
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Kobayashi, H., Sato, Y. & Egas, M. Males mate with females even after sperm depletion in the two-spotted spider mite. Exp Appl Acarol 86, 465–477 (2022). https://doi.org/10.1007/s10493-022-00706-x
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DOI: https://doi.org/10.1007/s10493-022-00706-x