Abstract
Crimean–Congo haemorrhagic fever (CCHF) is a severe tick-borne zoonotic disease in humans caused by CCHF virus. It has been observed in Bulgaria since 1952 and over the years more than 1600 cases have been reported in the country. Close contact with viraemic livestock was shown as one of the main causes of the infection. Detection of CCHF virus specific antibodies in livestock can be used as an indicator for virus circulation and risk assessment. CCHF seroprevalence was investigated in 843 cattle, 88 goats and 130 sheep, originating from all 28 districts of Bulgaria. CCHF virus-specific IgG antibodies were observed in 165 cattle (19.6, 95% CI 17.0–22.4%), in 20 goats (22.7, 95% CI 15.2–32.5%) and in 10 sheep (7.7, 95% CI 4.2–13.6%). The highest seroprevalence was detected in the district of Kardzhali, South Bulgaria (86.7, 95% CI 73.8–93.7%), a well-known focus of CCHF in humans. The other two districts with human CCHF cases, Blagoevgrad and Burgas, located in southwest and southeast Bulgaria, showed overall seroprevalences in livestock of 41.9% (95% CI 28.4–56.7%) and 31.3% (95% CI 22.2–42.1%), respectively. Seroprevalences in districts with no history of human CCHF cases varied between 55% (95% CI 39.8–69.3%) and 22.5% (95% CI 12.3–37.5%). These results suggest frequent CCHF virus infections even in regions without human CCHF cases and an enhanced risk of infection for humans in close contact with the infected livestock.
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Barthel R, Mohareb E, Younan R, Gladnishka T, Kalvatchev N, Moemen A, Mansour SS, Rossi C, Schoepp R, Christova I (2014) Seroprevalance of Crimean–Congo haemorrhagic fever in Bulgarian livestock. Biotechnol Biotechnol Equip 28:540–542
Bente DA, Forrester NL, Watts DM, McAuley AJ, Whitehouse CA, Bray M (2013) Crimean–Congo hemorrhagic fever: history, epidemiology, pathogenesis, clinical syndrome and genetic diversity. Anti-viral Res 100:159–189
Christova I, Gladnishka T, Taseva E, Kalvatchev N, Tsergouli K, Papa A (2013) Seroprevalence of Crimean–Congo hemorrhagic fever virus, Bulgaria. Emerg Infect Dis 19:177–179
Christova I, Panayotova E, Trifonova I, Taseva E, Hristova T, Ivanova V (2017) Country-wide seroprevalence studies on Crimean–Congo hemorrhagic fever and hantavirus infections in general population of Bulgaria. J Med Virol 89:1720–1725. https://doi.org/10.1002/jmv.24868
Fajs L, Humolli I, Saksida A, Knap N, Jelovṡek M, Korva M, Dedushaj I, Avṡic-Zupanc T (2014) Prevalence of Crimean–Congo hemorrhagic fever virus in healthy population, livestock and ticks in Kosovo. PLoS ONE 9(11):e110982
Lugaj A, Bërxhol K (2014) Serological survey of CCHFV in cattle in 10 regions of Albania. IMPACT Int J Res Appl Nat Soc Sci (IMPACT IJRANSS) 2:55–60
Maiga O, Sas MA, Rosenke K, Kamissoko B, Mertens M, Sogoba N, Traore A, Sangare M, Niang M, Schwan TG, Maiga HM, Traore SF, Feldmann H, Safronetz D, Groschup MH (2017) Serosurvey of Crimean–Congo hemorrhagic fever virus in cattle, Mali, West Africa. Am J Trop Med Hyg 96:1341–1345
Mertens M, Vatansever Z, Mrenoshki S, Krstevski K, Stefanovska J, Djadjovski I, Cvetkovikj I, Farkas R, Schuster I, Donnet F, Comtet L, Tordo N, Ben Mechlia M, Balkema-Buschmann A, Mitrov D, Groschup MH (2015) Circulation of Crimean–Congo hemorrhagic fever virus in the former Yugoslav Republic of Macedonia revealed by screening of cattle sera using a novel enzyme-linked immunosorbent assay. PLoS Negl Trop Dis 9(3):e0003519
Mertens M, Schuster I, Sas M, Vatansever Z, Hubalek Z, Güven E, Deniz A, Georgiev G, Peshev R, Groschup M (2016) Crimean–Congo hemorrhagic fever virus in Bulgaria and Turkey. Vector-borne Zoon Dis 16:619–623. https://doi.org/10.1089/vbz.2016.1944
Negredo A, de la Calle-Prieto F, Palencia-Herreion E, Mora-Rillo M, Astray-Mochales J, Sanchez-Seco MP, Bermeio Lopez E, Menarquez J, Fernandez-Cruz A, Sanchez-Artola B, Keough-Delgado E, Ramirez de Arellanto E, Lasaia F, Fraile JL, Ordpbas Gavin M, Martinez de la Gandara A, Lopez Perez L, Diaz-Diaz D, Lopez-Garcia MA, Delgado-Jimenez P, Martin-Quiros A, Trigo E, Figueira JC, Manzanares J, Rodriguez-Baena E, Garcia-Comas L, Rodriguez-Fraga O, Garcia-Arenzana N, Fernandez-Diaz MV, Corneio VM, Emmerich P, Schmidt-Chanasit J, Arribas JR, Crimean Congo Hemorrhagic Fever@Madrid Working Group (2017) Autochtonous Crimean–Congo hemorrhagic fever in Spain. N Engl J Med 377:154–161
Nekliudov M (1952) A case of hemorrhagic fever (Crimean). Savremenna Medicina 5:92–95
Panayotova E, Papa A, Trifonova I, Christova I (2016) Crimean–Congo hemorrhagic fever virus lineages Europe 1 and Europe 2 in Bulgarian ticks. Ticks Tick Borne Dis 7:1024–1028. https://doi.org/10.1016/j.ttbdis.2016.05.010
Papa A, Chaligiannis I, Kontana N, Sourba T, Tsioka K, Tsatsaris A, Sotiraki S (2014) A novel AP92-like Crimean–Congo hemorrhagic fever virus strain, Greece. Ticks Tick Borne Dis 5:590–593
Schuster I, Mertens M, Mrenoshki S, Staubach C, Mertens C, Bruning F, Wernike K, Hechinger S, Berxholi K, Mitrov D, Groschup MH (2016) Sheep and goats as indicator animals for the circulation of CCHFV in the environment. Exp Appl Acarol 68:337–346
Tuncer P, Yesilbag K, Alpay G, Dincer E, Girisgin AO, Aydin L et al (2014) Crimean–Congo hemorrhagic fever infection in domestic animals in Marmara region, Western Turkey. Ankara Univ Vet Fak Derg 61:49–53
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Christova, I., Panayotova, E., Groschup, M.H. et al. High seroprevalence for Crimean–Congo haemorrhagic fever virus in ruminants in the absence of reported human cases in many regions of Bulgaria. Exp Appl Acarol 75, 227–234 (2018). https://doi.org/10.1007/s10493-018-0258-7
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DOI: https://doi.org/10.1007/s10493-018-0258-7