Abstract
Trichoderma harzianum is a filamentous fungus that can act as a mycoparasite, saprophyte, or a plant symbiotic. It is widely used as a biological control agent against phytopathogenic fungi and can also be used for plant growth promotion and biofortification. Interaction between T. harzianum and phytopathogenic fungi involves mycoparasitism, competition, and antibiosis. Extracellular vesicles (EVs) have been described as presenting a central role in mechanisms of communication and interaction among fungus and their hosts. In this study, we characterized extracellular vesicles of T. harzianum produced during growth in the presence of glucose or S. sclerotiorum mycelia. A set of vesicular proteins was identified using proteomic approach, mainly presenting predicted signal peptides.
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Acknowledgements
The authors would like to thank the Laboratory of NanoTechnology, Universidade Federal de Goiás (Brazil), for their valuable help with the particle size and distribution analyses, and Professor Cirano José Ulhoa (Universidade Federal de Goiás, Brazil) for providing isolate TR274. Grabrielle Rosa is a recipient of the CAPES. (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) doctoral degree scholarship, and Eliane F. Noronha is a recipient of the CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) research fellowship.
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The authors acknowledge financial support from Brazilian funding agencies CNPq (National Council for Scientific and Technological Development), Fundação de Amparo a Pesquisa do Distrito Federal (FAP-DF), and Coordination for the Improvement of Higher Education Personnel (CAPES).
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Gabrielle Rosa Silva: Manuscript writing, data acquisition, data analyses. Felipe de Pina Cavalcanti: Data acquisition. Reynaldo Magalhães Melo: Data acquisition. Emilio Cintra: Data acquisition. Eliana Martins Lima: Data acquisition. Pedro Ricardo Vieira Hamann: manuscript writing, data analyses. Eliane Ferreira Noronha: manuscript writing, data analysis, and funding acquisition.
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Silva, G.R., de Pina Cavalcanti, F., Melo, R.M. et al. Extracellular vesicles from the mycoparasitic fungus Trichoderma harzianum. Antonie van Leeuwenhoek 117, 64 (2024). https://doi.org/10.1007/s10482-024-01958-w
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DOI: https://doi.org/10.1007/s10482-024-01958-w