Abstract
As part of a long-term study aiming to isolate and identify yeast species that inhabit the surface of leaves and fruits of native fine-aroma cacao in the department of Amazonas, Peru, we obtained multiple isolates of Hannaella species. Yeasts of the genus Hannaella are common inhabitants of the phyllosphere of natural and crop plants. On the basis of morphological, and physiological characteristics, and sequence analysis of the D1/D2 domains of the large subunit rRNA gene (LSU) and the internal transcribed spacer region (ITS), we identified five species of Hannaella from the phyllosphere of Peruvian cacao. Four have been previously described: H. phyllophila (isolates KLG-073, KLG-091), H. pagnoccae (KLG-076), H. sinensis (KLG-121), and H. taiwanensis (KLG-021). A fifth, represented by eight isolates (KLG-034, KLG-063, KLG-074, KLG-078, KLG-79, KLG-082, KLG-084, KLG-085), is not conspecific with any previously described Hannaella species, and forms the sister clade to H. surugaensis in the phylogenetic analysis. It has 2.6–3.9% (18–27 substitutions, 2–4 deletions, and 1–3 insertions in 610–938 bp-long alignments), and 9.8–10.0% nucleotide differences (37 substitutions and 14 insertions in 511–520 bp-long alignments) in the LSU and ITS regions, respectively, to H. surugaensis type strain, CBS 9426. Herein, the new species Hannaella theobromatis sp. nov. is described and characterised. The species epithet refers to its epiphytic ecology on its host Theobroma cacao.
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Data availability
All sequences generated in this study are publicly available under the NCBI Accession Numbers: OR086918-25, OR088043-50, OR088135-39, and OR088112-16 (For details, see Supplementary Table S1).
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Acknowledgements
The authors would like to thank to all members of the Plant Health Laboratory of the Instituto de Investigación para el Desarrollo Sustentable de Ceja de Selva from the National University Toribio Rodríguez de Mendoza de Amazonas, for their help during the conduction of this study.
Funding
This study was funded by the Project “Creación e Implementación del Centro de Investigación e Innovación Tecnológica en Cacao de la Universidad Nacional Toribio Rodríguez de Mendoza de Amazonas” (CEINCACAO), CUI N° 2315081; and by PROCIENCIA-CONCYTEC, Peru, under contract N° 057-2021-FONDECYT.
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JRD-V and MCA contributed to the study conception and design. Material preparation, data collection was performed by KJL-G. Data analyses were performed by KJL-G and JRD-V. The first draft of the manuscript was written by KJL-G, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Supplementary file 1. Supplementary Table S1. Passport and sequence information of isolates collected in this study and used in the phylogenetic study
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Supplementary file 2. Supplementary Table S2. Oxidation and assimilation tests of carbon-based compounds of the eigth strains of Hannaella theobromatis sp. nov.
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Llanos-Gómez, K.J., Aime, M.C. & Díaz-Valderrama, J.R. The surface of leaves and fruits of Peruvian cacao is home for several Hannaella yeast species, including the new species Hannaella theobromatis sp. nov.. Antonie van Leeuwenhoek 117, 43 (2024). https://doi.org/10.1007/s10482-024-01936-2
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DOI: https://doi.org/10.1007/s10482-024-01936-2