Abstract
The genus Gallaecimonas, proposed by Rodríguez-Blanco et al. (Int J Syst Evol Microbiol 60:504–509, 2010), is mainly isolated from marine environments. So far, only three species have been identified and characterized in this genus. In this study, a new Gallaecimonas strain named Q10T was isolated from the sediments of mangrove plant Kandelia obovate taken from Dapeng district, Shenzhen, China. Strain Q10T was a Gram-stain-negative, non-motile, strictly aerobic, rod-shaped bacterium, and grew with 0–8.0% (w/v) NaCl, at 10–45 °C and at pH 5.5–8.5. Phylogenetic analysis indicated that strain Q10T and the three Gallaecimonas species formed a clade in the tree, with 16S rRNA gene sequence similarities ranging from 96.0 to 97.0%. The major respiratory quinone is Q8. The polar lipids comprised aminolipid, aminophospholipid, diphosphatidylglycerol, glycolipid, phosphatidylethanolamine, phosphatidylglycerol, glycophospholipid and phospholipid. The predominant fatty acids are C16:0, C17:1ω8c, summed feature 3 (C16:1ω7c/C16:1ω6c), and iso-C16:0. The complete genome of strain Q10T is 3,836,841 bp with a G+C content of 62.6 mol%. The orthologous proteins analysis revealed 55 unique proteins in strain Q10T related to important biological processes, especially three frataxins related to iron–sulfur cluster assembly, which may play a pivotal role in environmental adaptability of this species. Based on polyphasic taxonomic data, strain Q10T is considered to represent a novel species within the genus Gallaecimonas, for which the name Gallaecimonas kandelia sp. nov. is proposed. The type strain is Q10T (=KCTC 92860T=MCCC 1K08421T). These results contribute to a better understanding of general features and taxonomy of the genus Gallaecimonas.
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Acknowledgements
We thank all the laboratory members for their critical reviews and comments on this manuscript, and we thank Professor Aharon Oren (The Hebrew University of Jerusalem, Israel) for his kind help in naming the novel species.
Funding
This work was financially supported by the Sustainable Development Project of Shenzhen Science and Technology Program (KCXFZ20211020165547010), and the Shenzhen Science and Technology Program (JCYJ20220530140813030).
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M.L. wrote the main manuscript text, and S.S.T and H.M.F collected the sediments samples. All authors reviewed the manuscript.
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Long, M., Tang, S., Fan, H. et al. Description and genomic characterization of Gallaecimonas kandeliae sp. nov., isolated from the sediments of mangrove plant Kandelia obovate. Antonie van Leeuwenhoek 116, 893–905 (2023). https://doi.org/10.1007/s10482-023-01851-y
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DOI: https://doi.org/10.1007/s10482-023-01851-y