Abstract
A Gram-negative, non-motile rod and strictly aerobic bacterium, designated as 18B16333T, was isolated from vertebral puncture tissue of a patient at Peking union medical college hospital in China. Growth occurred in NaCl concentrations of 0–1% (w/v) (optimum growth at 0% NaCl), at temperatures of 25–40 °C (optimum growth at 37 °C) and at pH 6.0–9.0 (optimum growth at pH 8.0). Diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine were the predominant polar lipids, and the major fatty acids were C16:0, C18:1 ω7c/C18:1 ω6c and C16:1 ω7c/C16:1 ω6c. Phylogenetic analysis based on 16S rRNA gene sequence comparisons indicated that strain 18B16333T was most closely related to Kingella potus CCUG 49773 T (97.3%, 16S rRNA gene sequence identity) and Neisseria bacilliformis CCUG 50858 T (96.8%). The ANI values between strain 18B16333T and the type strains K. potus CCUG 49773 T, N. bacilliformis CCUG 50858 T, Kingella kingae CCUG 352 T and Neisseria gonorrhoeae CCUG 26876 T were 77.3%, 79.1%, 72.1% and 75.4%, respectively. The dDDH values between strain 18B16333T and the four reference strains mentioned above were 24.8%, 26.9%, 24.2% and 20.7%. Further core gene analysis distinctively clustered strain 18B16333T with four Kingella species but not with Neisseria species. Based on the phenotypic, chemotaxonomic, and phylogenetic properties, strain 18B16333T represents a novel species of the genus Kingella, for which the name Kingella pumchi sp. nov. is proposed. The type strain is Kingella pumchi 18B16333T (= CICC 24913 T = CCUG 75125 T).
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Data availability
The GenBank accession number for the 16S rRNA gene sequence of strain 18B16333T is MW115420. The whole-genome shotgun project has been deposited at DDBJ/ENA/GenBank (Accession No. JAKOOW000000000) and the National Microbiology Data Center, and the accession number for the draft genome is (Accession No. NMDC60033523).
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This work was supported by the National Science and Technology Basic Resource Investigation Special Program of China (2019FY101200), the Beijing Nova Program (Z201100006820127), National High Level Hospital Clinical Research Funding (2022-PUMCH-B-074) and the Tsinghua University-Peking Union Medical College Hospital Initiative Scientific Research Program (20191080604).
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Xiao, M., Liu, R., Du, J. et al. Kingella pumchi sp. nov., an organism isolated from human vertebral puncture tissue. Antonie van Leeuwenhoek 116, 143–151 (2023). https://doi.org/10.1007/s10482-022-01786-w
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DOI: https://doi.org/10.1007/s10482-022-01786-w