Abstract
The waste and by-products of the soybean industry could be an economic source of nutrients to satisfy the high nutritional demands for the cultivation of lactic acid bacteria. The aims of this work were to maximize the biomass production of Lacticaseibacillus paracasei 90 (L90) in three culture media formulated from an effluent derived from soy protein concentrate production and to assess the effects these media have on the enzymatic activity of L90, together with their influence on its fermentation profile in milk. The presence of essential minerals and fermentable carbohydrates (sucrose, raffinose, and stachyose) in the effluent was verified. L90 reached high levels of microbiological counts (∼ 9 log cfu mL−1) and dry weight (> 1 g L−1) on the three optimized media. Enzymatic activities (lactate dehydrogenase and β-galactosidase) of L90, and its metabolism of lactose and citric acid, as well as lactic acid and pyruvic acid production in milk, were modified depending on the growth media. The ability of the L90 to produce the key flavour compounds (diacetyl and acetoin) was maintained or improved by growing in the optimized media in comparison with MRS.
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Funding
This work was financed by the Agencia Santafesina de Ciencia, Tecnología e Innovación de la provincia de Santa Fe-ASaCTeI (IO-2017-00036), the Agencia Nacional de Promoción Científica y Tecnológica-ANPCyT (PICT-2018-01334; PICT 2016-0597), and the Universidad Nacional del Litoral-UNL (PIC 50420150100059LI).
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Laboratory work M.V.B. and G.H.P; Optimization design L.V.C.; Volatile compounds determination I.V.W.; Minerals determination R.S.; Writing original draft G.H.P, M.V.B. and C.V.B; Project administration, Conceptualization and Funding acquisition G.H.P; E.R.H.; C.V.B. All authors contributed to writing the manuscript.
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Beret, M.V., Peralta, G.H., Vera-Candioti, L. et al. Culture media based on effluent derived from soy protein concentrate production for Lacticaseibacillus paracasei 90 biomass production: statistical optimisation, mineral characterization, and metabolic activities. Antonie van Leeuwenhoek 114, 2047–2063 (2021). https://doi.org/10.1007/s10482-021-01660-1
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DOI: https://doi.org/10.1007/s10482-021-01660-1