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Assessment of some metabolic activities and potential probiotic properties of eight Enterococcus bacteria isolated from white cheese microbiota

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Abstract

In the present study, eight strains of Enterococcus spp. were isolated from Turkish and Iranian white cheese test pieces. Enterococcus spp. strains were identified as Enterococcus faecium (6 strains) and E. faecalis (2) with 16S rDNA sequence analysis. All Enterococcus spp. strains showed susceptibility to the most of antibiotics tested in this investigation. The amount of produced acid (0.59–1.17%), hydrogen peroxide (0.65–3.91 µg/ml), and exopolysaccharide (252–362 mg/L) of these strains were determined. These strains possess the ability to inhibit Escherichia coli ATCC 35,218, Salmonella enteritidis ATCC 13,076, and Salmonella typhimurium MU 80. E. faecium RI53 and RI 42 strains were determined as the most resistant to acid (1.86 and 1.56 OD, respectively) and also exhibited high percentage of aggregation (54.1 and 51.7%, respectively). E. faecium RI 42 exhibited a higher growth viability in gastric and intestinal juice. E. faecium RI 53 and RI 42 are determined as optimal potential probiotic candidates for utilization in cheese preparations.

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Acknowledgements

The authors sincerely thank Gazi University, Faculty of Science, Department of Biology for the financial support.

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Authors and Affiliations

  1. Ostad Motahhari, Nikan Alley, 10 Sohrevardi Shomali Ave, 1567714413, Tehran, Iran

    Rasta Hajikhani

  2. Department of Biotechnology, Faculty of Science and Letters, Aksaray University, Aksaray, 68100, Turkey

    Derya Onal Darilmaz

  3. Department of Biology, Faculty of Science, Gazi University, Ankara, 06500, Turkey

    Zehra Nur Yuksekdag & Yavuz Beyatli

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  1. Rasta Hajikhani
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  2. Derya Onal Darilmaz
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  3. Zehra Nur Yuksekdag
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  4. Yavuz Beyatli
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YB and ZY conceived the study and analyzed the data. RH, DÖD and ZY performed molecular and microbiologic analyses. DÖD wrote the first draft of the report and all authors revised and approved the manuscript.

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Correspondence to Derya Onal Darilmaz.

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Hajikhani, R., Onal Darilmaz, D., Yuksekdag, Z. et al. Assessment of some metabolic activities and potential probiotic properties of eight Enterococcus bacteria isolated from white cheese microbiota. Antonie van Leeuwenhoek 114, 1259–1274 (2021). https://doi.org/10.1007/s10482-021-01599-3

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