Abstract
Pinus bungeana is one of indigenous trees in China and widely distributed in poor and arid regions for vegetation and industrial woody use. However, since a high-incidence disease threatens the growth of mature P. bungeana tree in the garden and in the plantation every year, the overwintering shoots were infected and died in the next spring with a ratio over 70%, but the cause was beyond understood. A total of 120 fungal isolates were separated from symptomatic twigs by histological isolation methods, including Pestalotiopsis spp., Fusarium spp., Trichothecium spp., Penicillium and some unknown fungal species. Pestalotiopsis spp. was dominant, accounting for 85%. Morphological observation under microcopy showed all Pestalotiopsis species are identical, and six isolations among them were randomly selected for pathogenicity tests. Fulfilling Koch’s postulates showed that all six isolates of Pestalotiopsis spp. were pathogens of twig blight, causing the same symptoms as observed in the field, while other non-Pestalotiopsis isolates were avirulent to P. bungeana twigs. Multi-gene (ITS, tub2 and TEF1) analysis and morphological observation revealed that all the six Pestalotiopsis isolates belonged to P. trachicarpicola. To our knowledge, this is the first study reporting P. trachicarpicola as the pathogens responsible for P. bungeana twig blight in China.
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Acknowledgements
We appreciate the support of Prof. Huang Lili for help collecting and investigation samples in field and Miss Ren Na for English reviewing.
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This study was supported by the National Key R&D Program of China (2017YFD0600103-4-2) and National Natural Science Committee (No .31670650).
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ZDY conceived the idea and designed the experiments. MQ and CXX carried out the research experiments and analyzed the results. MQ and ZDY drafted the manuscript. ZDY and QWC revised the manuscript. All authors read and approved the final version of the manuscript.
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Qi, M., Xie, CX., Chen, QW. et al. Pestalotiopsis trachicarpicola, a novel pathogen causes twig blight of Pinus bungeana (Pinaceae: Pinoideae) in China. Antonie van Leeuwenhoek 114, 1–9 (2021). https://doi.org/10.1007/s10482-020-01500-8
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DOI: https://doi.org/10.1007/s10482-020-01500-8