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Taxonomy of the yeast genus Vanderwaltozyma and proposal of Vanderwaltozyma meishanica sp. nov., Vanderwaltozyma huisunica sp. nov., and Vanderwaltozyma molinica sp. nov.

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Abstract

Using electrophoretic karyotyping, RAPD fingerprinting and phylogenetic analysis of ribosomal RNA gene sequences, twenty-six Vanderwaltozyma strains were studied. Out of 19 strains isolated in mountainous areas of Taiwan, eighteen strains were isolated from soil and one strain was isolated from the fruiting body of mushroom, six were identified as V. polyspora and three as V. verrucispora. Based on the results of a multigene sequence analysis (D1/D2, ITS and mitochondrial COX II gene) and DNA–DNA reassociation, three new ascosporic members of the genus Vanderwaltozyma are formally described: V. huisunica sp. nov. (GA1S06T = CBS 12250T = BCRC 23260T), V. meishanica sp. nov. (EN4S02T = CBS 12249T = BCRC 23255T) and V. molinica sp. nov. (GJ8S05T = CBS 12251T = BCRC 23264T), and the holotypes of these novel species are assigned as BCRC 23260T, BCRC 23255T and BCRC 23264T, respectively.

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Funding

This study was supported by the joint Taiwanese–Russian grant from the Ministry of Science and Technology, Executive Yuan, Taiwan (MOST 107-2923-B-007-001-MY3) and the Russian Foundation for Basic Research (RFBR 18-54-52002 MHT_a).

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Authors and Affiliations

  1. Department of Biological Science and Technology, China University of Science and Technology, Taipei, 11581, Taiwan

    Chin-Feng Chang

  2. Department of Applied Science, National Tsing Hua University, Nanda Campus, 521 Nanda Road, Hsinchu, 30014, Taiwan

    Yi-Ru Liu & Ching-Fu Lee

  3. State Research Institute of Genetics and Selection of Industrial Microorganisms of National Research Centre, Kurchatov Institute, Moscow, Russia

    Gennadi I. Naumov & Elena S. Naumova

  4. Institute of Analytical and Environmental Sciences, National Tsing Hua University, No. 101, Section 2, Kuang-Fu Road, Hsinchu, 30013, Taiwan

    Ching-Fu Lee

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  1. Chin-Feng Chang
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  2. Yi-Ru Liu
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  3. Gennadi I. Naumov
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  4. Elena S. Naumova
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  5. Ching-Fu Lee
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Contributions

CFC performed research and wrote the paper; YRL performed research; GIN and ESN editing and helped supervise the project; CFL designed study and supervised the project.

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Correspondence to Ching-Fu Lee.

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Chang, CF., Liu, YR., Naumov, G.I. et al. Taxonomy of the yeast genus Vanderwaltozyma and proposal of Vanderwaltozyma meishanica sp. nov., Vanderwaltozyma huisunica sp. nov., and Vanderwaltozyma molinica sp. nov.. Antonie van Leeuwenhoek 113, 663–676 (2020). https://doi.org/10.1007/s10482-019-01376-3

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