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Lysobacter oculi sp. nov., isolated from human Meibomian gland secretions

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Abstract

A Gram-stain negative, rod-shaped bacterial, catalase and oxidase positive strain (83-4T) that formed yellow colonies was isolated from human Meibomian gland secretions. Strain 83-4T belongs to the genus Lysobacter according to phylogenetic analysis based on 16S rRNA gene sequences. The DNA G+C content was 67.1 mol%. The circular genome was 2.6 Mb, which contained 2431 protein-coding sequences, 75 pseudogenes, 46 tRNAs, 3 rRNAs and 4 ncRNAs. A bacteriocin cluster and aryl polyene cluster were also found in the genome. The average nucleotide identity value was 79.6% between isolate 83-4T and the closely related type strain Lysobacter tolerans UM1T. The estimated DNA–DNA hybridization value between strain 83-4T and L. tolerans UM1T was 41.6%. Diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol were the major polar lipids. Iso-C15:0, iso-C11:0 3-OH, iso-C11:0 and summed feature 9 (iso-C17:1ω9c) were the major fatty acids. Ubiquinone (Q-8) was the only respiratory quinone. Therefore, based on the data of phylogenetic analysis, chemotaxonomical and biochemical analyses, it is concluded that strain 83-4T represents a novel species of the genus Lysobacter with the name of Lysobacter oculi sp. nov. The type strain is 83-4T (= CGMCC 1.13464T = NRBC 113451T).

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Acknowledgements

The authors would like to thank Jingnan Liang (Institute of Microbiology, CAS) for assistance with electron microscopy.

Funding

This research was supported by the National Science and Technology Major Project (2018ZX10101004-003-003) and the Ministry of Science and Technology of China (2010ZX09401-403).

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Author notes

  1. Hua Bai and Huibin Lv have contributed equally to this work.

Authors and Affiliations

  1. CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China

    Hua Bai

  2. CAS Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China

    Aihua Deng & Tingyi Wen

  3. Savaid Medical School, University of Chinese Academy of Sciences, Beijing, 100049, China

    Tingyi Wen

  4. Department of Ophthalmology, Peking University Third Hospital, Beijing Key Laboratory of Restoration of Damaged Ocular Nerve, Beijing, 100191, China

    Huibin Lv, Xiaodan Jiang & Xuemin Li

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  1. Hua Bai
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  2. Huibin Lv
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  3. Aihua Deng
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Correspondence to Xuemin Li or Tingyi Wen.

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The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain 83-4T is MG822667. The GenBank accession number for the whole-genome sequence of strain 83-4T is CP029556.

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Bai, H., Lv, H., Deng, A. et al. Lysobacter oculi sp. nov., isolated from human Meibomian gland secretions. Antonie van Leeuwenhoek 113, 13–20 (2020). https://doi.org/10.1007/s10482-019-01289-1

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